{"title":"评估烟草废料提取物作为木材防腐剂对抗木材腐朽真菌的潜力","authors":"Lin Liu, Junkai Tian, Ziyao Zhou, Chunwang Yang, Susu Yang, Kangkang Zhang, Yushan Yang, Jian Qiu","doi":"10.3390/f15071274","DOIUrl":null,"url":null,"abstract":"Utilizing conventional wood preservatives poses potential risks to ecosystems and human health. Therefore, the wood protection industry must develop alternatives that are both efficient and environmentally friendly. In this paper, industrial tobacco waste extracts were used as eco-friendly wood preservatives against wood decay fungi. Three major constituents in the extracts were identified via gas chromatography-mass spectrometry (GC-MS) and included nicotine, neophytadiene, and 2,7,11-cembratriene-4,6-diol. The antifungal activities of waste tobacco extracts and these three major constituents against wood decay fungi were tested. At a concentration of 40 mg/mL, the tobacco waste extract treated with 50% ethanol significantly inhibited the activity of wood decay fungi. This was because the extract contained nicotine as the primary active component and neophytadiene as a synergistic active component. Wood decay resistance tests were conducted on Pinus yunnanensis and Hevea brasiliensis treated with a 50% ethanol extract of tobacco waste at a concentration of 40 mg/mL. The mass losses of Pinus yunnanensis exposed to G. trabeum and T. versicolor were 4.11% and 5.03%, respectively, while the mass losses in Hevea brasiliensis exposed to G. trabeum and T. versicolor were 7.85% and 9.85%, respectively, which were classified as highly resistant. The acute ecotoxicity of the tobacco waste extract was assessed using a kinetic luminescent bacteria test with Aliivibrio fischeri, which revealed significantly lower acute toxicity than a commercial copper-based wood preservative. This study offers insights into high-value utilization of tobacco waste and advancement of natural wood preservatives.","PeriodicalId":505742,"journal":{"name":"Forests","volume":"25 13","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the Potentials of Tobacco Waste Extract as Wood Preservatives against Wood Decay Fungi\",\"authors\":\"Lin Liu, Junkai Tian, Ziyao Zhou, Chunwang Yang, Susu Yang, Kangkang Zhang, Yushan Yang, Jian Qiu\",\"doi\":\"10.3390/f15071274\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Utilizing conventional wood preservatives poses potential risks to ecosystems and human health. Therefore, the wood protection industry must develop alternatives that are both efficient and environmentally friendly. In this paper, industrial tobacco waste extracts were used as eco-friendly wood preservatives against wood decay fungi. Three major constituents in the extracts were identified via gas chromatography-mass spectrometry (GC-MS) and included nicotine, neophytadiene, and 2,7,11-cembratriene-4,6-diol. The antifungal activities of waste tobacco extracts and these three major constituents against wood decay fungi were tested. At a concentration of 40 mg/mL, the tobacco waste extract treated with 50% ethanol significantly inhibited the activity of wood decay fungi. This was because the extract contained nicotine as the primary active component and neophytadiene as a synergistic active component. Wood decay resistance tests were conducted on Pinus yunnanensis and Hevea brasiliensis treated with a 50% ethanol extract of tobacco waste at a concentration of 40 mg/mL. The mass losses of Pinus yunnanensis exposed to G. trabeum and T. versicolor were 4.11% and 5.03%, respectively, while the mass losses in Hevea brasiliensis exposed to G. trabeum and T. versicolor were 7.85% and 9.85%, respectively, which were classified as highly resistant. The acute ecotoxicity of the tobacco waste extract was assessed using a kinetic luminescent bacteria test with Aliivibrio fischeri, which revealed significantly lower acute toxicity than a commercial copper-based wood preservative. 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引用次数: 0
摘要
使用传统木材防腐剂会对生态系统和人类健康造成潜在风险。因此,木材保护行业必须开发既高效又环保的替代品。本文利用工业烟草废料提取物作为生态友好型木材防腐剂,对抗木材腐朽真菌。通过气相色谱-质谱法(GC-MS)鉴定了萃取物中的三种主要成分,包括烟碱、新茶二烯和 2,7,11-cembratriene-4,6-diol。测试了废弃烟草提取物和这三种主要成分对木材腐朽真菌的抗真菌活性。在浓度为 40 毫克/毫升时,用 50% 乙醇处理的烟草废提取物能显著抑制木材腐朽真菌的活性。这是因为萃取物中含有作为主要活性成分的烟碱和作为协同活性成分的新茶碱。用浓度为 40 毫克/毫升的烟草废料 50%乙醇提取物处理云南松和巴西红松,进行了木材抗腐烂试验。云南松暴露于 G. trabeum 和 T. versicolor 后的质量损失分别为 4.11% 和 5.03%,而巴西杉暴露于 G. trabeum 和 T. versicolor 后的质量损失分别为 7.85% 和 9.85%,属于高抗性。烟草废料提取物的急性生态毒性是通过阿里夫氏痢疾杆菌的动力学发光细菌试验进行评估的,结果表明其急性毒性明显低于商用铜基木材防腐剂。这项研究为烟草废料的高值化利用和天然木材防腐剂的发展提供了启示。
Evaluation of the Potentials of Tobacco Waste Extract as Wood Preservatives against Wood Decay Fungi
Utilizing conventional wood preservatives poses potential risks to ecosystems and human health. Therefore, the wood protection industry must develop alternatives that are both efficient and environmentally friendly. In this paper, industrial tobacco waste extracts were used as eco-friendly wood preservatives against wood decay fungi. Three major constituents in the extracts were identified via gas chromatography-mass spectrometry (GC-MS) and included nicotine, neophytadiene, and 2,7,11-cembratriene-4,6-diol. The antifungal activities of waste tobacco extracts and these three major constituents against wood decay fungi were tested. At a concentration of 40 mg/mL, the tobacco waste extract treated with 50% ethanol significantly inhibited the activity of wood decay fungi. This was because the extract contained nicotine as the primary active component and neophytadiene as a synergistic active component. Wood decay resistance tests were conducted on Pinus yunnanensis and Hevea brasiliensis treated with a 50% ethanol extract of tobacco waste at a concentration of 40 mg/mL. The mass losses of Pinus yunnanensis exposed to G. trabeum and T. versicolor were 4.11% and 5.03%, respectively, while the mass losses in Hevea brasiliensis exposed to G. trabeum and T. versicolor were 7.85% and 9.85%, respectively, which were classified as highly resistant. The acute ecotoxicity of the tobacco waste extract was assessed using a kinetic luminescent bacteria test with Aliivibrio fischeri, which revealed significantly lower acute toxicity than a commercial copper-based wood preservative. This study offers insights into high-value utilization of tobacco waste and advancement of natural wood preservatives.