对荷兰自生玫瑰种群进行基因分型以实现有效的原位基因保护管理

IF 3.1 3区 农林科学 Q1 HORTICULTURE
J. Buiteveld, Alisia Smolka, M. Smulders
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引用次数: 0

摘要

荷兰的大多数野蔷薇物种都属于蔷薇科(Rosa section Caninae),其他本土物种包括蔷薇(Rosa arvensis)(Synstylae 科)和蔷薇(Rosa spinosissima)(Pimpinellifoliae 科)。除了 Rosa canina 和 Rosa corymbifera 外,所有物种都很稀有,通常只有少量种群或零星个体。已为这些玫瑰制定了保护战略,重点是采用异地方法,包括克隆档案和种子果园,利用原始灌木进行无性繁殖。有效的采集管理旨在最大限度地保护遗传多样性,同时尽量减少重复的基因型。然而,狗牙根花卉的分类很复杂,因为存在物种杂交、不同倍性水平以及卡尼纳减数分裂导致的母系遗传等问题。它们还可以通过根吸器进行无性繁殖。为了评估荷兰野生玫瑰种群内部和之间的遗传结构、遗传多样性水平和克隆性,我们用 10 个高度多态性的微卫星标记对野生种群中的个体和异地基因库中的登录品进行了基因分型。分析结果显示,511 个采样个体中有 337 个不同的多焦点基因型(MLGs),其中一些 MLGs 在不同物种和地点之间共享。遗传结构分析表明,犬科中的非犬科物种之间存在明显的分群。MLGs 的地理分布表明,既有地方性分布,也有广泛分布。冗余分析从 244 个基因库登录样本中发现了 152 个不同的多基因组,表明冗余率为 38%。通过从不同物种组中选择 20-40 个个体的子集,优化了核心收集,以保留遗传多样性,并将冗余度降至最低。这项研究强调了遗传特征描述在指导狗尾草采样策略方面的价值。我们提出了一种分两步走的方法,可用于揭示克隆性和冗余性,并优化有性生殖和无性繁殖相结合的物种的核心采集,以最大限度地捕捉异地基因库中的基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genotyping of Autochthonous Rose Populations in the Netherlands for Effective Ex Situ Gene Conservation Management
Most wild rose species in the Netherlands belong to Rosa section Caninae (dogroses), with Rosa arvensis (section Synstylae) and Rosa spinosissima (section Pimpinellifoliae) as other indigenous species. All species are rare, often found in small populations or as scattered individuals, except for Rosa canina and Rosa corymbifera. Conservation strategies have been developed for these roses, with a focus on ex situ methods, including clonal archives and seed orchards, using vegetative propagation from the original shrubs. Efficient collection management aims at preservation of maximum genetic diversity with a minimum of duplicated genotypes. However, dogrose taxonomy is complex because of species hybridization, different ploidy levels, and their matroclinal inheritance due to Canina meiosis. They can also reproduce vegetatively through root suckers. In order to assess the genetic structure and the levels of genetic diversity and clonality within and among the wild rose populations in the Netherlands, we genotyped individuals in wild populations and accessions in the ex situ gene bank with 10 highly polymorphic microsatellite markers. The analysis revealed 337 distinct multilocus genotypes (MLGs) from 511 sampled individuals, with some MLGs shared across different species and sites. The genetic structure analysis showed distinct clusters separating non-dogrose species from the Caninae section. Geographic distribution of MLGs indicated both local and widespread occurrences. Redundancy analysis identified 152 distinct MLGs from 244 gene bank accessions, suggesting a 38% redundancy rate. Core collections were optimized to retain genetic diversity with minimal redundancy, selecting subsets of 20–40 individuals from different species groups. The study highlights the value of genetic characterization in guiding sampling strategies for dogroses. We propose a two-step approach that may be used to reveal clonality and redundancy and to optimize core collections of species that combine sexual and vegetative reproduction, to maximize genetic capture in ex situ gene banks.
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来源期刊
Horticulturae
Horticulturae HORTICULTURE-
CiteScore
3.50
自引率
19.40%
发文量
998
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