透视红姜体外组织培养及其对抗氧化活性的影响

D. Widyastuti, T. Nuringtyas, D. Santosa, A. Rohman
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引用次数: 0

摘要

红姜(Zingiber officinale var. rubrum)是印度尼西亚和马来西亚种植的生姜品种之一。与其他品种的生姜一样,红姜在印度尼西亚被广泛用作传统草药。由于花青素使根茎呈红色,因此红姜的抗氧化活性被认为高于其他生姜品种。由于栽培地点、栽培方法和加工方法的不同,红姜生物活性化合物的含量也不同。传统植物繁殖红姜的困难必须通过其他适当的技术来克服。体外组织培养是适合红姜栽培的潜在技术之一。这种技术可以培育出无病原体、具有生物活性化合物变异的生姜植株。体外组织培养还能提供较高的总酚类物质含量,这与生姜胼胝体中较高的抗氧化活性有关。在生姜胼胝体中产生最高抗氧化活性的最佳酵母提取物是 100 毫克/升。在此条件下,体外组织培养作为红生姜的替代栽培方法仍然很有前途,因为它能促进均匀、无菌和可控的小植株,并含有研究人员所呼吁的目标生物活性化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A perspective on the in vitro tissue culture of red ginger and its impact on antioxidant activity
Red ginger (Zingiber officinale var. rubrum) is one of the ginger varieties grown in Indonesia and Malaysia. Like other varieties of ginger, red ginger has been widely used as a traditional herbal medicine in Indonesia. The antioxidant activity of red ginger is suggested to be higher than other ginger varieties due to anthocyanin, which provide a red -coloured rhizome. The amount of red ginger bioactive compounds is different due to its cultivation place, cultivation method, and processing method. Difficulties of conventional vegetative propagation of red ginger have to be overcome with other appropriate techniques. One of the potential techniques as suitable methods for red ginger cultivation is in vitro tissue culture. This technique may produce pathogen-free ginger plantlets with a variation of bioactive compounds. In vitro tissue culture also provides higher total phenolic content related to higher antioxidant activity in elicited ginger callus. The best elicitor that yielded the highest antioxidant activity in ginger callus is yeast extract 100 mg/L. With that condition, in vitro tissue culture is still promising as an alternative cultivation method for red ginger to promote uniform, sterile and controlled plantlet with targeted bioactive compounds as the researcher urges.
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