转录组分析揭示了不同光质和光照时间诱导 "Kyoho "葡萄花青素合成的机制

IF 3.1 3区 农林科学 Q1 HORTICULTURE
Wu Sun, Yingying Yan, Zafarullah Muhammad, Guoqiang Zhang
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引用次数: 0

摘要

光对植物花青素的生物合成起着关键的调节作用。然而,夜间补充白光(W)、红光(R)、蓝光(B)和蓝光照射 3 小时、6 小时、9 小时和 12 小时(B3、B6、B9 和 B12)的葡萄皮花青素合成的分子基础尚不清楚。在本研究中,葡萄皮中的总花青素含量在不同光照(DL)和蓝光时间(DT)处理下有显著差异,其中 B9 处理的结果最好。对 RNA-seq 数据的分析表明,花青素合成途径中的关键基因 PAL、CHS、DFR、GT、CFIP、VIT_14s0068g00920、VIT_06s0009g02860 和 VIT_06s0004g08150 在夜间补光处理下显著上调、这导致苯丙氨酸生物合成、苯丙氨酸代谢、黄酮生物合成、黄酮和黄酮醇生物合成以及花青素生物合成途径明显表达,促进了花青素的积累。B 导致 PAL(VIT_08s0040g01710)、CFIP(VIT_13s0067g03820、VIT_13s0067g02870)和 DFR(VIT_18s0001g12800)的显著表达,这可能是 B 在葡萄皮中富集花青素的效果优于 W 的原因之一。DT 处理导致 GT(VIT_11s0052g01600)、过氧化物酶、VIT_09s0018g01190 和 VIT_11s0037g00570 的显著表达。此外,还发现许多 TFs,如 bHLH、MYB、ERF、WRKY、C2H2、MYB 相关和 NAC 参与了光调节下花青素的合成。这些结果为了解植物夜间补充光照对花青素积累的调控提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptomic Analyses Reveal the Mechanism by Which Different Light Qualities and Light Duration Induce Anthocyanin Biosynthesis in ‘Kyoho’ Grapes
The light plays a key role in regulating anthocyanin biosynthesis in plants. However, the molecular basis of anthocyanin synthesis in grape skins irradiated at night with supplemental white light (W), red light (R), blue light (B), and blue light for 3 h, 6 h, 9 h, and 12 h (B3, B6, B9, and B12) is not known. In the present study, the total anthocyanin content in grape skins was significant under different light (DL) and blue-light time (DT) treatments, and the best result was obtained with B9. Analysis of RNA-seq data determined that the key genes in the anthocyanin synthesis pathway, PAL, CHS, DFR, GT, CFIP, VIT_14s0068g00920, VIT_06s0009g02860, and VIT_06s0004g08150, were significantly up-regulated under night-time supplemental light treatments, which led to the significant expression of the phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, flavone and flavonol biosynthesis, and the anthocyanin biosynthesis pathway, which were significantly expressed, promoting the accumulation of anthocyanin. The B caused significant expression of PAL (VIT_08s0040g01710), CFIP (VIT_13s0067g03820, VIT_13s0067g02870) and DFR (VIT_18s0001g12800), which may be one of the reasons for the better effect of B compared to W anthocyanin enrichment in grape skins. The DT treatment resulted in significant expression of GT (VIT_11s0052g01600), Peroxidase, VIT_09s0018g01190, and VIT_11s0037g00570. In addition, many TFs such as bHLH, MYB, ERF, WRKY, C2H2, MYB-related, and NAC were found to be involved in the synthesis of anthocyanins under light regulation. These results provide new insights into plants’ nocturnal supplemental-light regulation of anthocyanin accumulation.
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来源期刊
Horticulturae
Horticulturae HORTICULTURE-
CiteScore
3.50
自引率
19.40%
发文量
998
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