Assessment of antiurolithiatic activity of some herbal-fractions using in vitro techniques

There is an extended inventory of medicinal plants used for urolithiasis due to their fewer side effects and because they contain copious phytochemicals that show advantageous effects in renal stones. The current research focuses on the in vitro evaluation of Trigonella foenum-graecum L., and Nigella sativa L., traditionally used in the treatment of urolithiasis by turbidity and titrimetric assay. Three different concentrations (1.0 mg/ml, 2.0 mg/ml, and 5.0 mg/ml) of the plant extracts were tested in each assay. The methanolic seed extracts of Trigonella foenum-graecum and Nigella sativa at 5.0 mg/ml exhibited a significant percentage of inhibition of calcium oxalate formation by turbidity method as well as percentage dissolution of calcium oxalate crystals by titrimetric method. The results of test groups displayed a near match to the standard drug Neeri used at 0.1mg/ml. This study has been primary evidence for Trigonella foenum-graecum and Nigella sativa as potential contributors to anti-urolithiasis.