通过脑内接种将挪威驯鹿CWD传染给绵羊,会导致不寻常的表型和朊病毒分布。

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Erez Harpaz, Federico Angelo Cazzaniga, Linh Tran, Tram T Vuong, Giuseppe Bufano, Øyvind Salvesen, Maiken Gravdal, Devin Aldaz, Julianna Sun, Sehun Kim, Luigi Celauro, Giuseppe Legname, Glenn C Telling, Michael A Tranulis, Sylvie L Benestad, Arild Espenes, Fabio Moda, Cecilie Ersdal
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引用次数: 0

摘要

慢性消耗性疾病(CWD)是一种影响颈鹿类动物的朊病毒疾病,早在20世纪60年代就已在北美出现,并于2016年在挪威出现。监测和研究显示,芬诺斯坎迪亚存在不同形式的慢性消耗性疾病:挪威驯鹿感染传染性慢性消耗性疾病,驼鹿和红鹿感染散发性慢性消耗性疾病。实验研究表明,NA CWD 的朊病毒可以感染各种物种,但迄今为止,还没有关于自然传播到非驯鹿物种的报道。对挪威CWD菌株的体外和实验动物研究表明,这些菌株与NA菌株不同。在这项研究中,我们描述了驯鹿 CWD 脑内传播给六只易感绵羊的情况。检测方法包括免疫组织化学(IHC)、免疫印迹(WB)、酶联免疫吸附试验(ELISA)、实时震荡诱导转换(RT-QuIC)和蛋白质错构周期扩增(PMCA)。在大脑中,灰质空泡化是有限的,而所有绵羊的白质都出现了空泡化。IHC和WB常规检测技术未能检测到朊病毒;但在除一只绵羊外的所有绵羊的中枢神经系统中,RT-QuIC和PMCA扩增技术都观察到了阳性播种活动。朊病毒在所有动物的淋巴结中都得到了扩增,主要是通过 RT-QuIC 扩增。此外,两个淋巴结通过 WB 检测呈阳性,一个通过 ELISA 检测呈阳性。这些研究结果表明,绵羊脑内接种后可传播驯鹿CWD朊病毒,导致不寻常的疾病表型和朊病毒分布,但可检测到的朊病毒数量较少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transmission of Norwegian reindeer CWD to sheep by intracerebral inoculation results in an unusual phenotype and prion distribution.

Chronic wasting disease (CWD), a prion disease affecting cervids, has been known in North America (NA) since the 1960s and emerged in Norway in 2016. Surveillance and studies have revealed that there are different forms of CWD in Fennoscandia: contagious CWD in Norwegian reindeer and sporadic CWD in moose and red deer. Experimental studies have demonstrated that NA CWD prions can infect various species, but thus far, there have been no reports of natural transmission to non-cervid species. In vitro and laboratory animal studies of the Norwegian CWD strains suggest that these strains are different from the NA strains. In this work, we describe the intracerebral transmission of reindeer CWD to six scrapie-susceptible sheep. Detection methods included immunohistochemistry (IHC), western blot (WB), enzyme-linked immunosorbent assay (ELISA), real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA). In the brain, grey matter vacuolation was limited, while all sheep exhibited vacuolation of the white matter. IHC and WB conventional detection techniques failed to detect prions; however, positive seeding activity with the RT-QuIC and PMCA amplification techniques was observed in the central nervous system of all but one sheep. Prions were robustly amplified in the lymph nodes of all animals, mainly by RT-QuIC. Additionally, two lymph nodes were positive by WB, and one was positive by ELISA. These findings suggest that sheep can propagate reindeer CWD prions after intracerebral inoculation, resulting in an unusual disease phenotype and prion distribution with a low amount of detectable prions.

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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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