单细胞分析显示 CD47 mRNA 表达与免疫细胞活化、抗病毒 Isgs 和细胞毒性相关。

IF 2.5 Q3 CELL BIOLOGY
Lamin B Cham, Miriam Rosas-Umbert, Lin Lin, Martin Tolstrup, Ole S Søgaard
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引用次数: 0

摘要

背景/目的:据报道,免疫细胞在感染过程中会上调 CD47,然而,CD47 在先天性和适应性免疫细胞中的作用仍不清楚:为了弥补这一知识空白,我们分析了我们的单细胞(sc)-RNA 数据集和其他公开的 sc-RNA 数据集,这些数据集来自健康对照、HIV-1 感染者(PWH)和 COVID-19 患者。我们根据 CD47 的低表达、中表达和高表达对每个免疫细胞进行了特征描述:我们的分析表明,CD47 高表达的 pDC 和单核细胞与 CD47 中表达和 CD47 低表达的细胞相比,IFN-α 调控基因、抗病毒干扰素刺激基因(ISGs)和 MHC-I 相关基因的表达相对较高。此外,CD47 高的 NK 和 CD8+ T 细胞表现出更高的抗病毒 ISGs 表达量,以及编码细胞毒性标记的基因,如颗粒酶 B、穿孔素、颗粒霉素、γ 干扰素和 NKG7。此外,CD47 高的 CD8+ T 细胞表达更高水平的 PD-1 和 LAG-3 基因。最后,我们发现,CD47 高的 B 细胞富集表达了参与细胞活化和体液反应的基因:总之,我们的分析表明,先天性和适应性免疫细胞表达较高的活化和功能基因特征,也表达较高的 CD47 水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single-Cell Analysis Reveals That CD47 mRNA Expression Correlates with Immune Cell Activation, Antiviral Isgs, and Cytotoxicity.

Background/aims: Immune cells are reported to upregulate CD47 during infection, however, the role of CD47 in innate and adaptive immune cells remains unclear.

Methods: To bridge this knowledge gap, we analysed our single cell (sc)-RNA dataset along with other publicly available sc-RNA datasets from healthy controls, people with HIV-1 (PWH) and COVID-19 patients. We characterized each immune cell based on low, intermediate, and high expression of CD47 .

Results: Our analyses revealed that CD47 high pDCs and monocytes exhibited relatively higher expression of IFN-α regulatory genes, antiviral interferon-stimulated genes (ISGs) and MHC-I associated genes compared to CD47 inter. and CD47 low cells. Furthermore, CD47 high NK and CD8+ T cells showed higher expression of antiviral ISGs, as well as genes encoding for cytotoxic markers like granzyme B, perforin, granulysin, interferon gamma and NKG7. Additionally, CD47 high CD8+ T cells expressed higher levels of PD-1 and LAG-3 genes. Lastly, we found that CD47 high B cells had enriched expression of genes involved in cell activation and humoral responses.

Conclusion: Overall, our analyses revealed that innate and adaptive immune cells expressing elevated activation and functional gene signatures also express higher CD47 levels.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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