Janni Nielsen, Johanne Lauritsen, Jannik N. Pedersen, Jan S. Nowak, Malthe K. Bendtsen, Giulia Kleijwegt, Kaija Lusser, Laia C. Pitarch, Julián V. Moreno, Matthias M. Schneider, Georg Krainer, Louise Goksøyr, Paul Khalifé, Sanne Simone Kaalund, Susana Aznar, Magnus Kjærgaard, Vita Sereikaité, Kristian Strømgaard, Tuomas P. J. Knowles, Morten Agertoug Nielsen, Adam F. Sander, Marina Romero-Ramos, Daniel E. Otzen
{"title":"针对细胞毒性α-突触核蛋白寡聚体的单克隆抗体的分子特性和诊断潜力","authors":"Janni Nielsen, Johanne Lauritsen, Jannik N. Pedersen, Jan S. Nowak, Malthe K. Bendtsen, Giulia Kleijwegt, Kaija Lusser, Laia C. Pitarch, Julián V. Moreno, Matthias M. Schneider, Georg Krainer, Louise Goksøyr, Paul Khalifé, Sanne Simone Kaalund, Susana Aznar, Magnus Kjærgaard, Vita Sereikaité, Kristian Strømgaard, Tuomas P. J. Knowles, Morten Agertoug Nielsen, Adam F. Sander, Marina Romero-Ramos, Daniel E. Otzen","doi":"10.1038/s41531-024-00747-6","DOIUrl":null,"url":null,"abstract":"<p>α-Synuclein (α-syn) accumulates as insoluble amyloid but also forms soluble α-syn oligomers (αSOs), thought to be even more cytotoxic than fibrils. To detect and block the unwanted activities of these αSOs, we have raised 30 monoclonal antibodies (mAbs) against different forms of αSOs, ranging from unmodified αSOs to species stabilized by lipid peroxidation products and polyphenols, αSOs formed by C-terminally truncated α-syn, and multivalent display of α-syn on capsid virus-like particles (cVLPs). While the mAbs generally show a preference for αSOs, they also bind fibrils, but to variable extents. Overall, we observe great diversity in the mAbs’ relative affinities for monomers and αSOs, varied requirements for the C-terminal extension of α-syn, and only a modest effect on α-syn fibrillation. Several mAbs show several orders of magnitude preference for αSOs over monomers in in-solution studies, while the commercial antibody MJF14 only bound 10-fold more strongly to αSOs than monomeric α-syn. Gratifyingly, seven mAbs almost completely block αSO permeabilization of membrane vesicles. Five selected mAbs identified α-syn-related pathologies like Lewy bodies (LBs) and Lewy Neurites, as well as Glial Cytoplasmic Inclusions in postmortem brains from people diagnosed for PD, dementia with LBs or multiple system atrophy, although to different extents. Three mAbs were particularly useful for pathological evaluation of postmortem brain human tissue, including early stages of PD. Although there was no straightforward connection between the mAbs’ biophysical and immunohistochemical properties, it is encouraging that this comprehensive collection of mAbs able to recognize different aggregated α-syn species in vitro also holds diagnostic potential.</p>","PeriodicalId":19706,"journal":{"name":"NPJ Parkinson's Disease","volume":null,"pages":null},"PeriodicalIF":6.7000,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular properties and diagnostic potential of monoclonal antibodies targeting cytotoxic α-synuclein oligomers\",\"authors\":\"Janni Nielsen, Johanne Lauritsen, Jannik N. Pedersen, Jan S. Nowak, Malthe K. Bendtsen, Giulia Kleijwegt, Kaija Lusser, Laia C. Pitarch, Julián V. Moreno, Matthias M. Schneider, Georg Krainer, Louise Goksøyr, Paul Khalifé, Sanne Simone Kaalund, Susana Aznar, Magnus Kjærgaard, Vita Sereikaité, Kristian Strømgaard, Tuomas P. J. Knowles, Morten Agertoug Nielsen, Adam F. Sander, Marina Romero-Ramos, Daniel E. Otzen\",\"doi\":\"10.1038/s41531-024-00747-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>α-Synuclein (α-syn) accumulates as insoluble amyloid but also forms soluble α-syn oligomers (αSOs), thought to be even more cytotoxic than fibrils. To detect and block the unwanted activities of these αSOs, we have raised 30 monoclonal antibodies (mAbs) against different forms of αSOs, ranging from unmodified αSOs to species stabilized by lipid peroxidation products and polyphenols, αSOs formed by C-terminally truncated α-syn, and multivalent display of α-syn on capsid virus-like particles (cVLPs). While the mAbs generally show a preference for αSOs, they also bind fibrils, but to variable extents. Overall, we observe great diversity in the mAbs’ relative affinities for monomers and αSOs, varied requirements for the C-terminal extension of α-syn, and only a modest effect on α-syn fibrillation. Several mAbs show several orders of magnitude preference for αSOs over monomers in in-solution studies, while the commercial antibody MJF14 only bound 10-fold more strongly to αSOs than monomeric α-syn. Gratifyingly, seven mAbs almost completely block αSO permeabilization of membrane vesicles. Five selected mAbs identified α-syn-related pathologies like Lewy bodies (LBs) and Lewy Neurites, as well as Glial Cytoplasmic Inclusions in postmortem brains from people diagnosed for PD, dementia with LBs or multiple system atrophy, although to different extents. Three mAbs were particularly useful for pathological evaluation of postmortem brain human tissue, including early stages of PD. 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Molecular properties and diagnostic potential of monoclonal antibodies targeting cytotoxic α-synuclein oligomers
α-Synuclein (α-syn) accumulates as insoluble amyloid but also forms soluble α-syn oligomers (αSOs), thought to be even more cytotoxic than fibrils. To detect and block the unwanted activities of these αSOs, we have raised 30 monoclonal antibodies (mAbs) against different forms of αSOs, ranging from unmodified αSOs to species stabilized by lipid peroxidation products and polyphenols, αSOs formed by C-terminally truncated α-syn, and multivalent display of α-syn on capsid virus-like particles (cVLPs). While the mAbs generally show a preference for αSOs, they also bind fibrils, but to variable extents. Overall, we observe great diversity in the mAbs’ relative affinities for monomers and αSOs, varied requirements for the C-terminal extension of α-syn, and only a modest effect on α-syn fibrillation. Several mAbs show several orders of magnitude preference for αSOs over monomers in in-solution studies, while the commercial antibody MJF14 only bound 10-fold more strongly to αSOs than monomeric α-syn. Gratifyingly, seven mAbs almost completely block αSO permeabilization of membrane vesicles. Five selected mAbs identified α-syn-related pathologies like Lewy bodies (LBs) and Lewy Neurites, as well as Glial Cytoplasmic Inclusions in postmortem brains from people diagnosed for PD, dementia with LBs or multiple system atrophy, although to different extents. Three mAbs were particularly useful for pathological evaluation of postmortem brain human tissue, including early stages of PD. Although there was no straightforward connection between the mAbs’ biophysical and immunohistochemical properties, it is encouraging that this comprehensive collection of mAbs able to recognize different aggregated α-syn species in vitro also holds diagnostic potential.
期刊介绍:
npj Parkinson's Disease is a comprehensive open access journal that covers a wide range of research areas related to Parkinson's disease. It publishes original studies in basic science, translational research, and clinical investigations. The journal is dedicated to advancing our understanding of Parkinson's disease by exploring various aspects such as anatomy, etiology, genetics, cellular and molecular physiology, neurophysiology, epidemiology, and therapeutic development. By providing free and immediate access to the scientific and Parkinson's disease community, npj Parkinson's Disease promotes collaboration and knowledge sharing among researchers and healthcare professionals.