红/绿蓝藻色素从藻氰基生物素异构为藻氰基生物素。

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Protein Science Pub Date : 2024-08-01 DOI:10.1002/pro.5132
Hiroki Hoshino, Keita Miyake, Keiji Fushimi, Rei Narikawa
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引用次数: 0

摘要

蓝藻生物色素(CBCR)是蓝藻特有的光感受器,与植物色素关系密切。大多数 CBCR 含有保守的半胱氨酸残基,称为标准 Cys,而有些 CBCR 在 DXCF 基序中还含有额外的半胱氨酸残基,称为第二 Cys,因此被归类为 DXCF CBCR。它们通常会经历这样一个过程:结合植物花青素(PCB),然后将其异构化为植物花青素(PVB)。相反,具有保守的 Trp 残基但没有第二个 Cys 的 CBCR 被称为扩展红/绿(XRG)CBCR。典型的 XRG CBCR 结合多氯联苯时不会发生多氯联苯到 PVB 的异构化,表现出红绿可逆的光电转换。我们发现了带有 Cys 残基而非保守的 Trp 残基的新型 XRG CBCR。这些新型 XRG CBCRs 具有将 PCB 异构为 PVB 的能力,显示出绿色/茶色的可逆光电转换。通过基于序列和结构的比较以及诱变实验,我们确定了包括 Cys 残基在内的三个氨基酸残基对促进 PCB 到 PVB 的异构化至关重要。这项研究拓展了我们对 XRG CBCRs 多样性的认识,凸显了 CBCRs 显著的分子可塑性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Red/green cyanobacteriochromes acquire isomerization from phycocyanobilin to phycoviolobilin.

Cyanobacteriochromes (CBCRs) are unique cyanobacteria-specific photoreceptors that share a distant relation with phytochromes. Most CBCRs contain conserved cysteine residues known as canonical Cys, while some CBCRs have additional cysteine residues called second Cys within the DXCF motif, leading to their classification as DXCF CBCRs. They typically undergo a process where they incorporate phycocyanobilin (PCB) and subsequently isomerize it to phycoviolobilin (PVB). Conversely, CBCRs with conserved Trp residues and without the second Cys are called extended red/green (XRG) CBCRs. Typical XRG CBCRs bind PCB without undergoing PCB-to-PVB isomerization, displaying red/green reversible photoconversion, and there are also atypical CBCRs that exhibit diverse photoconversions. We discovered novel XRG CBCRs with Cys residue instead of the conserved Trp residue. These novel XRG CBCRs exhibited the ability to isomerize PCB to PVB, displaying green/teal reversible photoconversion. Through sequence- and structure-based comparisons coupled with mutagenesis experiments, we identified three amino acid residues, including the Cys residue, crucial for facilitating PCB-to-PVB isomerization. This research expands our understanding of the diversity of XRG CBCRs, highlighting the remarkable molecular plasticity of CBCRs.

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来源期刊
Protein Science
Protein Science 生物-生化与分子生物学
CiteScore
12.40
自引率
1.20%
发文量
246
审稿时长
1 months
期刊介绍: Protein Science, the flagship journal of The Protein Society, is a publication that focuses on advancing fundamental knowledge in the field of protein molecules. The journal welcomes original reports and review articles that contribute to our understanding of protein function, structure, folding, design, and evolution. Additionally, Protein Science encourages papers that explore the applications of protein science in various areas such as therapeutics, protein-based biomaterials, bionanotechnology, synthetic biology, and bioelectronics. The journal accepts manuscript submissions in any suitable format for review, with the requirement of converting the manuscript to journal-style format only upon acceptance for publication. Protein Science is indexed and abstracted in numerous databases, including the Agricultural & Environmental Science Database (ProQuest), Biological Science Database (ProQuest), CAS: Chemical Abstracts Service (ACS), Embase (Elsevier), Health & Medical Collection (ProQuest), Health Research Premium Collection (ProQuest), Materials Science & Engineering Database (ProQuest), MEDLINE/PubMed (NLM), Natural Science Collection (ProQuest), and SciTech Premium Collection (ProQuest).
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