Ilse Ekman , Anna-Mari Schroderus , Tytti Vuorinen , Mikael Knip , Riitta Veijola , Jorma Toppari , Jorma Ilonen , Johanna Lempainen , Tuure Kinnunen
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引用次数: 0
摘要
巨细胞病毒（CMV）感染会对免疫系统，尤其是记忆 T 细胞产生终身影响。然而，早期巨细胞病毒感染对婴儿 T 细胞表型和功能的影响，尤其是对儿童期 T 细胞纵向变化的影响还没有详细的研究。我们使用高维流式细胞术分析了早期（小于 6 个月）感染 CMV 的儿童外周血 CD8+ 和 CD4+ T 细胞的表型和功能。研究人员收集了6个月和6岁时CMV IgG-血清阳性（CMV+）儿童的样本，并与CMV-血清阴性（CMV-）儿童的样本进行了比较。早期CMV感染会导致T细胞发生多种改变。这些变化包括：6 个月大时，CD27+ 早期效应记忆 CD8+ 和 CD4+ T 细胞和 CD27- 晚期效应记忆 CD8+ 和 CD4+ T 细胞中的 CD28 表达下调，CD57 表达上调。在这些变化中，只有高度分化的晚期效应记忆区的变化在 6 岁时仍然存在。生命早期的 CMV 感染对发育中的 CD8+ 和 CD4+ 记忆 T 细胞区有明显的影响。它似乎会诱发暂时性和长期性的改变，这可能会影响免疫系统一生的功能。
The effect of early life cytomegalovirus infection on the immune profile of children
Cytomegalovirus (CMV) infection has a life-long impact on the immune system, particularly on memory T cells. However, the effect of early life CMV infection on the phenotype and functionality of T cells in infants and especially longitudinal changes occurring during childhood have not been explored in detail.
The phenotype and functionality of peripheral blood CD8+ and CD4+ T cells from children infected with CMV in early life (< 6 months of age) was analyzed using high-dimensional flow cytometry. Samples from CMV IgG-seropositive (CMV+) children were collected at 6 months and 6 years of age and compared to samples from CMV-seronegative (CMV-) children.
Early life CMV infection caused multiple alterations within T cells. These include downregulation of CD28 expression and upregulation of CD57 expression within both CD27+ early and CD27- late effector memory CD8+ and CD4+ T-cells at 6 months of age. Of these changes, only alterations within the highly differentiated late effector memory compartment persisted at the age of 6 years.
Early life CMV-infection has a distinct impact on developing CD8+ and CD4+ memory T cell compartments. It appears to induce both temporary as well as longer-lasting alterations, which may affect the functionality of the immune system throughout life.
期刊介绍：
Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.
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