Fatimah Muhammad Ballah, M Nazmul Hoque, Md Saiful Islam, Golam Mahbub Faisal, Al-Muksit Mohammad Taufiquer Rahman, Mst Minara Khatun, Marzia Rahman, Jayedul Hassan, Md Tanvir Rahman
{"title":"从食品处理人员中分离出的耐甲氧西林生物膜金黄色葡萄球菌菌株的基因组研究。","authors":"Fatimah Muhammad Ballah, M Nazmul Hoque, Md Saiful Islam, Golam Mahbub Faisal, Al-Muksit Mohammad Taufiquer Rahman, Mst Minara Khatun, Marzia Rahman, Jayedul Hassan, Md Tanvir Rahman","doi":"10.1155/2024/5516117","DOIUrl":null,"url":null,"abstract":"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm-producing and multidrug-resistant (MDR) <i>S. aureus</i> MTR_BAU_H1 strain through whole-genome sequencing (WGS). The <i>S. aureus</i> MTR_BAU_H1 strain was isolated from food handlers' hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the <i>S. aureus</i> MTR_BAU_H1 strain is a strong biofilm-former and carries both antimicrobial resistance (e.g., methicillin resistance; <i>mec</i>A, beta-lactam resistance; <i>bla</i>Z and tetracycline resistance; <i>tet</i>C) and virulence (e.g., <i>sea</i>, <i>tsst</i>, and <i>PVL</i>) genes. The genome of the <i>S. aureus</i> MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first-ever WGS-based study of a strong biofilm-producing and MDR <i>S. aureus</i> strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals.</p>","PeriodicalId":9007,"journal":{"name":"BioMed Research International","volume":"2024 ","pages":"5516117"},"PeriodicalIF":2.6000,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11283335/pdf/","citationCount":"0","resultStr":"{\"title\":\"Genomic Insights of a Methicillin-Resistant Biofilm-Producing <i>Staphylococcus aureus</i> Strain Isolated From Food Handlers.\",\"authors\":\"Fatimah Muhammad Ballah, M Nazmul Hoque, Md Saiful Islam, Golam Mahbub Faisal, Al-Muksit Mohammad Taufiquer Rahman, Mst Minara Khatun, Marzia Rahman, Jayedul Hassan, Md Tanvir Rahman\",\"doi\":\"10.1155/2024/5516117\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. 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Genomic Insights of a Methicillin-Resistant Biofilm-Producing Staphylococcus aureus Strain Isolated From Food Handlers.
Methicillin-resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm-producing and multidrug-resistant (MDR) S. aureus MTR_BAU_H1 strain through whole-genome sequencing (WGS). The S. aureus MTR_BAU_H1 strain was isolated from food handlers' hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the S. aureus MTR_BAU_H1 strain is a strong biofilm-former and carries both antimicrobial resistance (e.g., methicillin resistance; mecA, beta-lactam resistance; blaZ and tetracycline resistance; tetC) and virulence (e.g., sea, tsst, and PVL) genes. The genome of the S. aureus MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first-ever WGS-based study of a strong biofilm-producing and MDR S. aureus strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals.
期刊介绍:
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.