评估马匹妊娠过程中的 IL-6 细胞因子家族

IF 2.5 3区 医学 Q3 IMMUNOLOGY
Carleigh E. Fedorka, Kirsten E. Scoggin, Hossam El-Sheikh Ali, Mats H. T. Troedsson
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引用次数: 0

摘要

简介白细胞介素(IL)-6 细胞因子家族由一个共同的受体亚基(gp130)组成,但在不同的生理活动中发挥着不同但重叠的作用,包括调节急性期反应以及效应T细胞群和调节T细胞群之间的平衡--这两种细胞因子在妊娠的成功成熟中都发挥着作用。为此,我们对妊娠 120 天、180 天、300 天和 330 天的母马绒毛膜和子宫内膜(n = 4/阶段)以及妊娠 45 天的绒毛膜(n = 4)和绝经期子宫内膜(n = 3)进行了 RNA 测序。除了IL-6受体(IL-6R)和共同受体亚基gp130外,还评估了IL-6细胞因子家族成员的表达水平,包括睫状神经营养因子(CNTF)、心脏营养素1(CT-1)、心脏营养素样细胞因子因子1(CLCF1)、galectin-10、oncostatin M(OSM)、IL-6、-11和-27。此外,还评估了IL-6的外周浓度:结果:在绒毛膜中,IL-6、IL-11、CNTF、CLCF1、OSM和CT-1的表达存在差异。在子宫内膜中,妊娠年龄影响着IL-11、CNTF和CT-1的表达。循环中IL-6的浓度在妊娠120天时达到最高,在妊娠45天、180天、300天和330天时浓度较低。在整个妊娠期,IL-6R 和 gp130 的表达都发生了变化:总之,IL-6 细胞因子家族的成员似乎在整个马妊娠过程中不断波动,在比较单个成员时会发现不同的表达谱。此外,在比较绒毛膜、子宫内膜和血液循环时也发现了不同的表达谱,这表明细胞因子的功能具有组织特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluating the IL-6 Family of Cytokines Throughout Equine Gestation

Evaluating the IL-6 Family of Cytokines Throughout Equine Gestation

Introduction

The interleukin (IL)-6 family of cytokines is grouped by a common receptor subunit (gp130), but functions in distinct but overlapping physiological activities, including regulation of acute phase reaction and the balance between effector and regulatory T cell populations—both of which play a role in successful pregnancy maturation.

Methods

Here, we aim to assess the expression profiles of members of the IL-6 cytokine family throughout equine gestation. To do so, RNA Sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300, and 330 days of gestation (n = 4/stage), as well as 45-day chorioallantois (n = 4) and diestrus endometrium (n = 3). Expression levels of members of the IL-6 cytokine family including ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), galectin-10, oncostatin M (OSM), and IL-6, -11, and -27 were evaluated in addition to the receptors for IL-6 (IL-6R) and the common receptor subunit gp130. Additionally, peripheral concentration of IL-6 was assessed.

Results

In the chorioallantois, differential expression of IL-6, IL-11, CNTF, CLCF1, OSM, and CT-1 was noted. In the endometrium, the gestational age of pregnancy impacted the expression of IL-11, CNTF, and CT-1. Circulatory IL-6 concentrations reached their highest concentrations at 120 days, with lesser concentrations noted at 45, 180, 300, and 330 days. Both IL-6R and gp130 altered in expression throughout equine gestation.

Conclusion

In conclusion, members of the IL-6 cytokine family appear to fluctuate constantly throughout equine pregnancy, with varying expression profiles noted when comparing individual members. Additionally, different expression profiles were noted when comparing chorioallantois, endometrium, and circulation, indicating that the function of the cytokine is tissue-specific.

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来源期刊
CiteScore
6.20
自引率
5.60%
发文量
314
审稿时长
2 months
期刊介绍: The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.
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