Carleigh E. Fedorka, Kirsten E. Scoggin, Hossam El-Sheikh Ali, Mats H. T. Troedsson
{"title":"评估马匹妊娠过程中的 IL-6 细胞因子家族","authors":"Carleigh E. Fedorka, Kirsten E. Scoggin, Hossam El-Sheikh Ali, Mats H. T. Troedsson","doi":"10.1111/aji.13910","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>The interleukin (IL)-6 family of cytokines is grouped by a common receptor subunit (gp130), but functions in distinct but overlapping physiological activities, including regulation of acute phase reaction and the balance between effector and regulatory T cell populations—both of which play a role in successful pregnancy maturation.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Here, we aim to assess the expression profiles of members of the IL-6 cytokine family throughout equine gestation. To do so, RNA Sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300, and 330 days of gestation (<i>n</i> = 4/stage), as well as 45-day chorioallantois (<i>n</i> = 4) and diestrus endometrium (<i>n</i> = 3). Expression levels of members of the IL-6 cytokine family including ciliary neurotrophic factor (<i>CNTF</i>), cardiotrophin 1 (<i>CT-1</i>), cardiotrophin-like cytokine factor 1 (<i>CLCF1</i>), galectin-10, oncostatin M (<i>OSM</i>), and <i>IL-6, -11</i>, and -<i>27</i> were evaluated in addition to the receptors for IL-6 (<i>IL-6R</i>) and the common receptor subunit <i>gp130</i>. Additionally, peripheral concentration of IL-6 was assessed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>In the chorioallantois, differential expression of <i>IL-6, IL-11, CNTF, CLCF1, OSM</i>, and <i>CT-1</i> was noted. In the endometrium, the gestational age of pregnancy impacted the expression of <i>IL-11, CNTF</i>, and <i>CT-1</i>. Circulatory IL-6 concentrations reached their highest concentrations at 120 days, with lesser concentrations noted at 45, 180, 300, and 330 days. Both <i>IL-6R</i> and <i>gp130</i> altered in expression throughout equine gestation.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>In conclusion, members of the IL-6 cytokine family appear to fluctuate constantly throughout equine pregnancy, with varying expression profiles noted when comparing individual members. Additionally, different expression profiles were noted when comparing chorioallantois, endometrium, and circulation, indicating that the function of the cytokine is tissue-specific.</p>\n </section>\n </div>","PeriodicalId":7665,"journal":{"name":"American Journal of Reproductive Immunology","volume":"92 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aji.13910","citationCount":"0","resultStr":"{\"title\":\"Evaluating the IL-6 Family of Cytokines Throughout Equine Gestation\",\"authors\":\"Carleigh E. Fedorka, Kirsten E. Scoggin, Hossam El-Sheikh Ali, Mats H. T. Troedsson\",\"doi\":\"10.1111/aji.13910\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>The interleukin (IL)-6 family of cytokines is grouped by a common receptor subunit (gp130), but functions in distinct but overlapping physiological activities, including regulation of acute phase reaction and the balance between effector and regulatory T cell populations—both of which play a role in successful pregnancy maturation.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Here, we aim to assess the expression profiles of members of the IL-6 cytokine family throughout equine gestation. To do so, RNA Sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300, and 330 days of gestation (<i>n</i> = 4/stage), as well as 45-day chorioallantois (<i>n</i> = 4) and diestrus endometrium (<i>n</i> = 3). Expression levels of members of the IL-6 cytokine family including ciliary neurotrophic factor (<i>CNTF</i>), cardiotrophin 1 (<i>CT-1</i>), cardiotrophin-like cytokine factor 1 (<i>CLCF1</i>), galectin-10, oncostatin M (<i>OSM</i>), and <i>IL-6, -11</i>, and -<i>27</i> were evaluated in addition to the receptors for IL-6 (<i>IL-6R</i>) and the common receptor subunit <i>gp130</i>. Additionally, peripheral concentration of IL-6 was assessed.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>In the chorioallantois, differential expression of <i>IL-6, IL-11, CNTF, CLCF1, OSM</i>, and <i>CT-1</i> was noted. In the endometrium, the gestational age of pregnancy impacted the expression of <i>IL-11, CNTF</i>, and <i>CT-1</i>. Circulatory IL-6 concentrations reached their highest concentrations at 120 days, with lesser concentrations noted at 45, 180, 300, and 330 days. Both <i>IL-6R</i> and <i>gp130</i> altered in expression throughout equine gestation.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>In conclusion, members of the IL-6 cytokine family appear to fluctuate constantly throughout equine pregnancy, with varying expression profiles noted when comparing individual members. 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Evaluating the IL-6 Family of Cytokines Throughout Equine Gestation
Introduction
The interleukin (IL)-6 family of cytokines is grouped by a common receptor subunit (gp130), but functions in distinct but overlapping physiological activities, including regulation of acute phase reaction and the balance between effector and regulatory T cell populations—both of which play a role in successful pregnancy maturation.
Methods
Here, we aim to assess the expression profiles of members of the IL-6 cytokine family throughout equine gestation. To do so, RNA Sequencing was performed on chorioallantois and endometrium of mares at 120, 180, 300, and 330 days of gestation (n = 4/stage), as well as 45-day chorioallantois (n = 4) and diestrus endometrium (n = 3). Expression levels of members of the IL-6 cytokine family including ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), cardiotrophin-like cytokine factor 1 (CLCF1), galectin-10, oncostatin M (OSM), and IL-6, -11, and -27 were evaluated in addition to the receptors for IL-6 (IL-6R) and the common receptor subunit gp130. Additionally, peripheral concentration of IL-6 was assessed.
Results
In the chorioallantois, differential expression of IL-6, IL-11, CNTF, CLCF1, OSM, and CT-1 was noted. In the endometrium, the gestational age of pregnancy impacted the expression of IL-11, CNTF, and CT-1. Circulatory IL-6 concentrations reached their highest concentrations at 120 days, with lesser concentrations noted at 45, 180, 300, and 330 days. Both IL-6R and gp130 altered in expression throughout equine gestation.
Conclusion
In conclusion, members of the IL-6 cytokine family appear to fluctuate constantly throughout equine pregnancy, with varying expression profiles noted when comparing individual members. Additionally, different expression profiles were noted when comparing chorioallantois, endometrium, and circulation, indicating that the function of the cytokine is tissue-specific.
期刊介绍:
The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.