{"title":"用于快速检测纯水中次氯酸盐的自组装纳米探针及其在活细胞、食品和环境系统中的应用。","authors":"","doi":"10.1016/j.talanta.2024.126597","DOIUrl":null,"url":null,"abstract":"<div><p>As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO<sup>−</sup>) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe <strong>Nano-SJD</strong>, effectively detect ClO<sup>−</sup> in both food samples and living cells. The small molecular probe <strong>SJD</strong> with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, <strong>SJD</strong> was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe <strong>Nano-SJD</strong>. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO<sup>−</sup> in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO<sup>−</sup> in real-water samples. Additionally, the robust fluorescent imaging behavior of <strong>Nano-SJD</strong> for visualization of ClO<sup>−</sup> in living cells highlights its broad potential in biosystem applicability.</p></div>","PeriodicalId":435,"journal":{"name":"Talanta","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A self-assembled nanoprobe for rapid detection of hypochlorite in pure water and its application in living cells, food and environmental systems\",\"authors\":\"\",\"doi\":\"10.1016/j.talanta.2024.126597\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO<sup>−</sup>) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe <strong>Nano-SJD</strong>, effectively detect ClO<sup>−</sup> in both food samples and living cells. The small molecular probe <strong>SJD</strong> with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, <strong>SJD</strong> was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe <strong>Nano-SJD</strong>. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO<sup>−</sup> in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO<sup>−</sup> in real-water samples. Additionally, the robust fluorescent imaging behavior of <strong>Nano-SJD</strong> for visualization of ClO<sup>−</sup> in living cells highlights its broad potential in biosystem applicability.</p></div>\",\"PeriodicalId\":435,\"journal\":{\"name\":\"Talanta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.6000,\"publicationDate\":\"2024-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Talanta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0039914024009767\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0039914024009767","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
A self-assembled nanoprobe for rapid detection of hypochlorite in pure water and its application in living cells, food and environmental systems
As an important ROS species participating in various physiological and pathological processes, high level of hypochlorite (ClO−) poses significant health and safety concerns, necessitating efficient detection methods. Herein, this study introduces a water-soluble fluorescent nanoprobe Nano-SJD, effectively detect ClO− in both food samples and living cells. The small molecular probe SJD with N, N-dimethylthiocarbamyl (DMTC) as recognition moiety was constructed based on a naphthalene derivative. To further improve the water solubility, SJD was assembled with an amphiphilic copolymer (mPEG-DSPE) to prepare a water soluble fluorescent nanoprobe Nano-SJD. Fortunately, the nanoprobe preserves the excellent properties of small molecules and performs very well optical response to ClO− in aqueous solution, possessing the advantages including ultra-rapid response (within 1 s), minimal interference, low detection limits (0.39 μM) and good pH stability. What's more important, we have also developed smartphone-compatible test paper strips for convenient on-site detection of ClO− in real-water samples. Additionally, the robust fluorescent imaging behavior of Nano-SJD for visualization of ClO− in living cells highlights its broad potential in biosystem applicability.
期刊介绍:
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.