畸形翅病毒基因A型和B型不会在天真蜜蜂宿主体内引起不同的免疫反应

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Amanda M. Norton, Gabriele Buchmann, Alyson Ashe, Owen T. Watson, Madeleine Beekman, Emily J. Remnant
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引用次数: 0

摘要

畸形翅病毒(Iflavirus aladeformis)(Picornavirales: Iflaviridae),俗称畸形翅病毒(DWV),与瓦罗阿破坏者安德森和特鲁曼(Varroa destructor Anderson and Trueman)(Mesostigmata: Varroidae)一起,是造成蜜蜂(Apis mellifera L. [Hymenoptera: Apidae])死亡的主要因素。病毒和螨虫几乎遍布全球,因此很难将两者的影响区分开来。随着时间的推移,两种主要的 DWV 基因型(DWV-A 和 DWV-B)的流行率发生了变化,这导致两种毒株可能会引起宿主不同的免疫反应。在此,我们利用对螨虫和病毒均无免疫反应的蜜蜂种群,研究蜜蜂是否对 DWV 基因型表现出不同的免疫反应。我们通过反转录定量 PCR(RT-qPCR)检测了 19 个免疫基因的表达,并分析了实验性注射 DWV-A 和 DWV-B 后的小 RNA。我们没有发现任何证据表明 DWV-A 和 DWV-B 在蜜蜂体内引起了不同的免疫反应。在DWV感染过程中,RNA干扰基因上调,小干扰RNA(siRNA)反应与病毒载量成正比,但并不能抑制DWV的积累。对DWV的siRNA反应弱于对另一种蜜蜂病原体Triatovirus nigereginacellulae(Picornavirales: Dicistroviridae;黑蜂王浆病毒)的反应,这表明DWV在逃避宿主抗病毒防御方面相对更强。没有证据表明 DWV 可产生源自病毒的 Piwi-interacting RNA(piRNA)。与以前的研究不同,在没有破坏者病毒的情况下,我们没有发现 DWV 有免疫抑制作用的证据。总之,我们的研究结果加深了我们对 DWV 在蜜蜂体内引起的免疫效应的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Deformed wing virus genotypes A and B do not elicit immunologically different responses in naïve honey bee hosts
Iflavirus aladeformis (Picornavirales: Iflaviridae), commonly known as deformed wing virus(DWV), in association with Varroa destructor Anderson and Trueman (Mesostigmata: Varroidae), is a leading factor associated with honey bee (Apis mellifera L. [Hymenoptera: Apidae]) deaths. The virus and mite have a near global distribution, making it difficult to separate the effect of one from the other. The prevalence of two main DWV genotypes (DWV‐A and DWV‐B) has changed over time, leading to the possibility that the two strains elicit a different immune response by the host. Here, we use a honey bee population naïve to both the mite and the virus to investigate if honey bees show a different immunological response to DWV genotypes. We examined the expression of 19 immune genes by reverse transcription quantitative PCR (RT‐qPCR) and analysed small RNA after experimental injection with DWV‐A and DWV‐B. We found no evidence that DWV‐A and DWV‐B elicit different immune responses in honey bees. RNA interference genes were up‐regulated during DWV infection, and small interfering RNA (siRNA) responses were proportional to viral loads yet did not inhibit DWV accumulation. The siRNA response towards DWV was weaker than the response to another honey bee pathogen, Triatovirus nigereginacellulae (Picornavirales: Dicistroviridae; black queen cell virus), suggesting that DWV is comparatively better at evading host antiviral defences. There was no evidence for the production of virus‐derived Piwi‐interacting RNAs (piRNAs) in response to DWV. In contrast to previous studies, and in the absence of V. destructor, we found no evidence that DWV has an immunosuppressive effect. Overall, our results advance our understanding of the immunological effect that DWV in isolation elicits in honey bees.
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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