Tamir Partosh, Michael Davidovitz, Noa Firer, Gur Pines
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引用次数: 0
摘要
Harmonia axyridis (Pallas),俗称亚洲瓢虫,是亚洲的原生昆虫物种,曾被有意引入不同地区进行生物防治。然而,它在原释放地以外的广泛存在表明其具有高度入侵性。在这项研究中,我们利用 CRISPR-Cas9 方法实现了对 H. axyridis 的精确基因组编辑。具体来说,我们以H. axyridis中的两个基因--漆酶2和猩红--为目标,敲除这两个基因在其他昆虫中的同源基因会出现明显的表型变化。敲除漆酶2不仅会导致早期可检测到的表型,还会导致致死。然而,我们通过破坏猩红色基因,成功地建立了一个有生命力且遗传稳定的突变体群,从而产生了白眼甲虫。我们的研究结果有助于扩大对 H. axyridis 遗传操作的认识,并为其在未来研究中的潜力以及在生物控制和入侵物种管理中的实际应用提供了见解。
Stable clustered regularly interspaced short palindromic repeats‐based genome editing in Harmonia axyridis
Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. In this study, we utilized the CRISPR‐Cas9 approach to achieve precise genome editing in H. axyridis. Specifically, we targeted two genes in H. axyridis, laccase2 and scarlet, knockdown of which orthologues in other insects showed visible phenotypic changes. The knockout laccase2 resulted not only in an early‐detectable phenotype but also in lethality. However, we successfully established a viable and genetically stable mutant colony by disrupting the scarlet gene, resulting in beetles with white eyes. Our findings contribute to the expanding knowledge of genetic manipulation in H. axyridis and provide insights into its potential for future research and practical applications for biocontrol and invasive species management.
期刊介绍:
The Journal of Applied Entomology publishes original articles on current research in applied entomology, including mites and spiders in terrestrial ecosystems.
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