全转录组 m6A 甲基化和代谢组分析揭示了锰胁迫下水稻根系的调控网络

IF 4.5 2区 生物学 Q2 ENVIRONMENTAL SCIENCES
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引用次数: 0

摘要

与其他谷物相比,水稻()对锰(Mn)胁迫的耐受性更高。然而,人们对水稻耐锰的调控机制仍然知之甚少。在这项研究中,先用 1.0 mM MnCl 处理水稻栽培品种的幼苗 10 天,然后收集根部样本进行全转录组甲基腺苷(mA)甲基化和代谢组分析。在额外的锰存在下,我们发现了 2050 个明显改变的 mA 峰和 2549 个差异表达基因(DEGs)。这些 DEG 与 MAPK 信号转导、钙信号转导和过氧化物等关键信号通路有关。其中,282 个 DEGs 出现了不同的 mA 甲基化峰,包括 29 个转录因子,表明它们可能是锰毒性反应的关键上游调节因子。此外,代谢组学研究表明,水稻根系在锰胁迫下发生了相当大的代谢变化,尤其是在嘌呤代谢、氨基酸生物合成和甘油磷脂代谢途径方面。近一半的代谢物是脂类或类脂化合物,这表明它们在信号转导和膜生物生成方面具有潜在功能。这些发现有助于更好地了解水稻根系中有助于耐受锰胁迫的调控网络。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Transcriptome-wide m6A methylation and metabolomic analysis reveal regulatory networks in rice roots under manganese stress

Rice (Oryza sativa) has a higher tolerance to manganese (Mn) stress than other cereals. However, the regulatory mechanisms governing Mn tolerance in rice remain poorly understood. In this work, seedlings of the rice cultivar Nipponbare were treated with 1.0 mM MnCl2 for 10 days before root samples were collected for transcriptome-wide N6-methyladenosine (m6A) methylation and metabolome profiling. In the presence of extra Mn, we identified 2050 significantly modified m6A peaks and 2549 differentially expressed genes (DEGs). These DEGs were linked to key signaling pathways such as MAPK signaling, calcium signaling, and peroxides. Among these, 282 DEGs showed differential m6A methylation peaks, including 29 transcription factors, indicating they might be key upstream regulators of the Mn toxicity response. Furthermore, metabolomic research indicated considerable metabolic alterations in rice roots under Mn stress, notably in purine metabolism, amino acid biosynthesis, and glycerophospholipid metabolic pathways. Almost half of the metabolites were lipids or lipid-like compounds, indicating a potential function in signal transduction and membrane biogenesis. The findings lead to a better understanding of regulatory networks in rice roots that aid in Mn stress tolerance.

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来源期刊
Environmental and Experimental Botany
Environmental and Experimental Botany 环境科学-环境科学
CiteScore
9.30
自引率
5.30%
发文量
342
审稿时长
26 days
期刊介绍: Environmental and Experimental Botany (EEB) publishes research papers on the physical, chemical, biological, molecular mechanisms and processes involved in the responses of plants to their environment. In addition to research papers, the journal includes review articles. Submission is in agreement with the Editors-in-Chief. The Journal also publishes special issues which are built by invited guest editors and are related to the main themes of EEB. The areas covered by the Journal include: (1) Responses of plants to heavy metals and pollutants (2) Plant/water interactions (salinity, drought, flooding) (3) Responses of plants to radiations ranging from UV-B to infrared (4) Plant/atmosphere relations (ozone, CO2 , temperature) (5) Global change impacts on plant ecophysiology (6) Biotic interactions involving environmental factors.
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