用于兔子后巩膜加固的生物材料的可行性和安全性。

Wen-Fei Zhang, Bin-Han Li, Zi-Bo Liu, Tai-Ran Peng, Rong-Ping Dai, Zhi-Kun Yang, Yue-Lin Wang, Zhuo Xiong, Chan Wu, Ting Zhang, An-Quan Xue, You-Xin Chen
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引用次数: 0

摘要

目的: 探讨生物材料用于家兔后巩膜加固(PSR)的可行性和安全性。 方法: 对新鲜牛心包和猪颅内膜进行脱细胞和基因素交联,然后测试其机械性能、缝合保持强度和稳定性。使用处理过的生物材料对 24 只兔眼进行了 PSR 手术。在 PSR 手术前后(1 周、1 个月、3 个月、6 个月)定期进行眼科检查。为了评估效果,还进行了超声波、屈光度和光学相干断层扫描(OCT)检查。 结果: 与吉尼平交联牛心包(Gen-BP)(21.29 ± 13.29 Mpa)相比,吉尼平交联猪颅内膜(Gen-PE)(34.85 ± 3.67 Mpa,P < 0.01)的弹性模量更接近吉尼平交联人巩膜。没有出现与材料直接相关的并发症或毒性反应。观察到毛细血管增生、炎症细胞浸润和胶原纤维沉积,PSR 后 I 型胶原纤维含量增加。总体而言,PSR 后不同时间点的脉络膜厚度明显增厚,分别为 96.84 ± 21.08μm、96.72 ± 22.00μm、90.90 ± 16.57μm、97.28 ± 14.74μm。Gen-PE组的变化与总体数据基本一致。在材料性能方面,Gen-PE 组比 Gen-BP 组具有更显著的优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The feasibility and safety of biomaterials for posterior scleral reinforcement in rabbits.

To explore the feasibility and safety of biomaterials for posterior scleral reinforcement (PSR) in rabbits. Decellularization and genipin crosslink were applied to the fresh bovine pericardium and porcine endocranium, and then mechanical properties, suture retention strength, and stability were tested. PSR operation was performed on 24 rabbit eyes using treated biological materials. Ophthalmic examination was performed regularly before and after PSR operation (1 week, 1 month, 3 months, 6 months). To evaluate the effectiveness, A ultrasound, diopter, and optical coherence tomography were conducted. General condition, fundus photograph, and pathological examination were recorded to evaluate the safety. Compared with genipin crosslinked bovine pericardium (Gen-BP) (21.29 ± 13.29 Mpa), genipin crosslinked porcine endocranium (Gen-PE) (34.85 ± 3.67 Mpa,P< 0.01) showed a closer elastic modulus to that of genipin crosslinked human sclera. There were no complications or toxic reactions directly related to the materials. Capillary hyperplasia, inflammatory cell infiltration, and collagen fiber deposition were observed, and the content of type I collagen fibers increased after PSR. Overall, the choroidal thickness of treated eyes was significantly thickened at different time points after PSR, which were 96.84 ± 21.08 μm, 96.72 ± 22.00 μm, 90.90 ± 16.57 μm, 97.28 ± 14.74 μm, respectively. The Gen-PE group showed changes that were almost consistent with the overall data. Gen-BP and Gen-PE are safe biological materials for PSR. The Gen-PE group demonstrated more significant advantages over the Gen-BP group in terms of material properties.

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