Chengkuizengella axinellae sp.

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Yea-Lin Moon, Kyung Hyun Kim, Jin-Sook Park
{"title":"Chengkuizengella axinellae sp.","authors":"Yea-Lin Moon,&nbsp;Kyung Hyun Kim,&nbsp;Jin-Sook Park","doi":"10.1007/s10482-024-01998-2","DOIUrl":null,"url":null,"abstract":"<div><p>A Gram-stain-positive, strictly aerobic, creamy-white colored, endospore-forming and non-motile rods strain, designated as strain 2205SS18-9<sup>T</sup>, was isolated from a marine sponge, <i>Axinella</i> sp. collected from Seopseom Island, Republic of Korea. Optimal growth of strain 2205SS18-9<sup>T</sup> was observed at 25–30 °C, pH 6.5–7.0, and in the presence of 3.0% (w/v) NaCl. Cells were oxidase-positive and catalase-negative. Negative for nitrate reduction and indole production. Phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealed that strain 2205SS18-9<sup>T</sup> formed a distinct phyletic lineage in the genus <i>Chengkuizengella</i>, and it was most closely related to <i>Chengkuizengella marina</i> YPA3-1-1<sup>T</sup> and <i>Chengkuizengella sediminis</i> J15A17<sup>T</sup> with 97.1 and 96.6% 16S rRNA gene sequence similarities, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between strain 2205SS18-9<sup>T</sup> and <i>Chengkuizengella marina</i> YPA3-1-1<sup>T</sup> were 79.0 and 21.6%, respectively. The genomic DNA G + C content was 34.1%. The genome harbors a number of host-adhesion and transporter genes, suggested that strain 2205SS18-9<sup>T</sup> may interact with its sponge host as a symbiont. Menaquinone-7 was the sole isoprenoid quinone and antieiso-C<sub>15:0</sub> (28.5%), iso-C<sub>16:0</sub> (25.8%), C<sub>16:1</sub> <i>ω</i>7c alcohol (15.0%), and iso-C<sub>15:0</sub> (11.2%) were detected as the major fatty acids. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, and an unidentified lipid. The cell-wall peptidoglycan contained lysine, alanine, glutamate, and aspartate. Based on these analyses, strain 2205SS18-9<sup>T</sup> represents a novel species of the genus <i>Chengkuizengella</i>, for which the name <i>Chengkuizengella axinellae</i> sp. nov. is proposed. The type strain is 2205SS18-9<sup>T</sup> (= KACC 23238<sup>T</sup> = LMG 33063<sup>T</sup>).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella\",\"authors\":\"Yea-Lin Moon,&nbsp;Kyung Hyun Kim,&nbsp;Jin-Sook Park\",\"doi\":\"10.1007/s10482-024-01998-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A Gram-stain-positive, strictly aerobic, creamy-white colored, endospore-forming and non-motile rods strain, designated as strain 2205SS18-9<sup>T</sup>, was isolated from a marine sponge, <i>Axinella</i> sp. collected from Seopseom Island, Republic of Korea. Optimal growth of strain 2205SS18-9<sup>T</sup> was observed at 25–30 °C, pH 6.5–7.0, and in the presence of 3.0% (w/v) NaCl. Cells were oxidase-positive and catalase-negative. Negative for nitrate reduction and indole production. Phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealed that strain 2205SS18-9<sup>T</sup> formed a distinct phyletic lineage in the genus <i>Chengkuizengella</i>, and it was most closely related to <i>Chengkuizengella marina</i> YPA3-1-1<sup>T</sup> and <i>Chengkuizengella sediminis</i> J15A17<sup>T</sup> with 97.1 and 96.6% 16S rRNA gene sequence similarities, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between strain 2205SS18-9<sup>T</sup> and <i>Chengkuizengella marina</i> YPA3-1-1<sup>T</sup> were 79.0 and 21.6%, respectively. The genomic DNA G + C content was 34.1%. The genome harbors a number of host-adhesion and transporter genes, suggested that strain 2205SS18-9<sup>T</sup> may interact with its sponge host as a symbiont. Menaquinone-7 was the sole isoprenoid quinone and antieiso-C<sub>15:0</sub> (28.5%), iso-C<sub>16:0</sub> (25.8%), C<sub>16:1</sub> <i>ω</i>7c alcohol (15.0%), and iso-C<sub>15:0</sub> (11.2%) were detected as the major fatty acids. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, and an unidentified lipid. The cell-wall peptidoglycan contained lysine, alanine, glutamate, and aspartate. Based on these analyses, strain 2205SS18-9<sup>T</sup> represents a novel species of the genus <i>Chengkuizengella</i>, for which the name <i>Chengkuizengella axinellae</i> sp. nov. is proposed. The type strain is 2205SS18-9<sup>T</sup> (= KACC 23238<sup>T</sup> = LMG 33063<sup>T</sup>).</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":\"117 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-07-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-024-01998-2\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-01998-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

从大韩民国西奥普森岛采集的海洋海绵 Axinella sp.中分离出一株革兰氏染色阳性、严格需氧、乳白色、内生孢子形成且不运动的菌株,命名为 2205SS18-9T 菌株。2205SS18-9T 菌株的最佳生长温度为 25-30℃,pH 值为 6.5-7.0,存在 3.0%(w/v)的氯化钠。细胞氧化酶阳性,过氧化氢酶阴性。硝酸盐还原和吲哚生成阴性。基于 16S rRNA 基因和全基因组序列的系统进化分析表明,菌株 2205SS18-9T 在 Chengkuizengella 属中形成了一个独特的藻系,它与 Chengkuizengella marina YPA3-1-1T 和 Chengkuizengella sediminis J15A17T 的亲缘关系最密切,16S rRNA 基因序列相似度分别为 97.1% 和 96.6%。2205SS18-9T 与 Chengkuizengella marina YPA3-1-1T 的平均核苷酸相同度和数字 DNA-DNA 杂交值分别为 79.0% 和 21.6%。基因组 DNA G + C 含量为 34.1%。基因组中含有大量宿主粘附和转运基因,这表明 2205SS18-9T 菌株可能作为共生体与海绵宿主相互作用。萘醌-7是唯一的异戊二烯醌,主要脂肪酸为反-异-C15:0(28.5%)、异-C16:0(25.8%)、C16:1 ω7c醇(15.0%)和异-C15:0(11.2%)。极性脂质包括二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、两种不明氨基磷脂和一种不明脂质。细胞壁肽聚糖含有赖氨酸、丙氨酸、谷氨酸和天门冬氨酸。根据上述分析,2205SS18-9T 菌株代表了 Chengkuizengella 属的一个新菌种,并将其命名为 Chengkuizengella axinellae sp.模式菌株为 2205SS18-9T (= KACC 23238T = LMG 33063T)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella

Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella

Chengkuizengella axinellae sp. nov., a symbiotic bacterium isolated from a marine sponge of the genus Axinella

A Gram-stain-positive, strictly aerobic, creamy-white colored, endospore-forming and non-motile rods strain, designated as strain 2205SS18-9T, was isolated from a marine sponge, Axinella sp. collected from Seopseom Island, Republic of Korea. Optimal growth of strain 2205SS18-9T was observed at 25–30 °C, pH 6.5–7.0, and in the presence of 3.0% (w/v) NaCl. Cells were oxidase-positive and catalase-negative. Negative for nitrate reduction and indole production. Phylogenetic analyses based on the 16S rRNA gene and whole-genome sequences revealed that strain 2205SS18-9T formed a distinct phyletic lineage in the genus Chengkuizengella, and it was most closely related to Chengkuizengella marina YPA3-1-1T and Chengkuizengella sediminis J15A17T with 97.1 and 96.6% 16S rRNA gene sequence similarities, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between strain 2205SS18-9T and Chengkuizengella marina YPA3-1-1T were 79.0 and 21.6%, respectively. The genomic DNA G + C content was 34.1%. The genome harbors a number of host-adhesion and transporter genes, suggested that strain 2205SS18-9T may interact with its sponge host as a symbiont. Menaquinone-7 was the sole isoprenoid quinone and antieiso-C15:0 (28.5%), iso-C16:0 (25.8%), C16:1 ω7c alcohol (15.0%), and iso-C15:0 (11.2%) were detected as the major fatty acids. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, and an unidentified lipid. The cell-wall peptidoglycan contained lysine, alanine, glutamate, and aspartate. Based on these analyses, strain 2205SS18-9T represents a novel species of the genus Chengkuizengella, for which the name Chengkuizengella axinellae sp. nov. is proposed. The type strain is 2205SS18-9T (= KACC 23238T = LMG 33063T).

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信