Israt Islam , Badriya Mubashshira , Spencer Mark Mondol , Otun Saha , M. Shaminur Rahman , Afroza Khan , Amiruzzaman , Md. Mizanur Rahaman
{"title":"从孟加拉国感染伤口中分离出的耐多药 ST 491 鲍曼不动杆菌 BD20 的流行病学模式和基因组学见解：关于三类β内酰胺酶基因的共存。","authors":"Israt Islam , Badriya Mubashshira , Spencer Mark Mondol , Otun Saha , M. Shaminur Rahman , Afroza Khan , Amiruzzaman , Md. Mizanur Rahaman","doi":"10.1016/j.jgar.2024.07.009","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Multidrug-resistant (MDR) <em>Acinetobacter baumannii</em> is a major issue within healthcare facilities in Bangladesh due to its frequent association with hospital-acquired infections. In this study we report on a carbapenem-resistant draft genome sequence of an <em>A. baumannii</em> BD20 sample isolated from an infected wound in Bangladesh.</p></div><div><h3>Methods</h3><p><em>A. baumannii</em> BD20 was isolated from an infected burn wound. Whole-genome sequencing was carried out and annotated using PGAP and Prokka. Sequence type, antimicrobial resistance genes, virulence factor genes, and metal resistance genes were investigated. Core genome multilocus sequence typing–based phylogenomic analysis between <em>A. baumannii</em> BD20 and 213 <em>A. baumannii</em> strains retrieved from the NCBI GenBank database was performed using the BacWGSTdb 2.0 server.</p></div><div><h3>Results</h3><p><em>A. baumannii</em> BD20 (MLST 491) was resistant to all the antibiotics tested, except for colistin and polymyxin B. Along with many other antibiotic resistance genes, the isolate harbored three classes of beta lactamase–producing genes: <em>bla</em><sub>GES-11</sub> (class A), <em>bla</em><sub>OXA-69</sub> (class D), <em>bla</em><sub>ADC-10</sub> (class C), and <em>bla</em><sub>ADC-11</sub> (class C). Additionally, the strain carried several virulence genes and metal resistance determinants, which may contribute to its increased virulence. Core genome MLST–based phylogenomic analysis revealed that <em>A. baumannii</em> BD20 was closely related to another ST491 strain isolated from Singapore.</p></div><div><h3>Conclusions</h3><p>The findings of this study underscore the growing challenge of MDR <em>A. baumannii</em>, emphasizing the need for vigilant surveillance and infection-control measures in healthcare settings in order to address these emerging threats effectively.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"38 ","pages":"Pages 327-331"},"PeriodicalIF":3.7000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524001395/pdfft?md5=c90865ae9f0839867ab7ef6c308f03b5&pid=1-s2.0-S2213716524001395-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Epidemiological pattern and genomic insights into multidrug-resistant ST491 Acinetobacter baumannii BD20 isolated from an infected wound in Bangladesh: Concerning co-occurrence of three classes of beta lactamase genes\",\"authors\":\"Israt Islam , Badriya Mubashshira , Spencer Mark Mondol , Otun Saha , M. Shaminur Rahman , Afroza Khan , Amiruzzaman , Md. Mizanur Rahaman\",\"doi\":\"10.1016/j.jgar.2024.07.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>Multidrug-resistant (MDR) <em>Acinetobacter baumannii</em> is a major issue within healthcare facilities in Bangladesh due to its frequent association with hospital-acquired infections. In this study we report on a carbapenem-resistant draft genome sequence of an <em>A. baumannii</em> BD20 sample isolated from an infected wound in Bangladesh.</p></div><div><h3>Methods</h3><p><em>A. baumannii</em> BD20 was isolated from an infected burn wound. Whole-genome sequencing was carried out and annotated using PGAP and Prokka. Sequence type, antimicrobial resistance genes, virulence factor genes, and metal resistance genes were investigated. Core genome multilocus sequence typing–based phylogenomic analysis between <em>A. baumannii</em> BD20 and 213 <em>A. baumannii</em> strains retrieved from the NCBI GenBank database was performed using the BacWGSTdb 2.0 server.</p></div><div><h3>Results</h3><p><em>A. baumannii</em> BD20 (MLST 491) was resistant to all the antibiotics tested, except for colistin and polymyxin B. Along with many other antibiotic resistance genes, the isolate harbored three classes of beta lactamase–producing genes: <em>bla</em><sub>GES-11</sub> (class A), <em>bla</em><sub>OXA-69</sub> (class D), <em>bla</em><sub>ADC-10</sub> (class C), and <em>bla</em><sub>ADC-11</sub> (class C). Additionally, the strain carried several virulence genes and metal resistance determinants, which may contribute to its increased virulence. Core genome MLST–based phylogenomic analysis revealed that <em>A. baumannii</em> BD20 was closely related to another ST491 strain isolated from Singapore.</p></div><div><h3>Conclusions</h3><p>The findings of this study underscore the growing challenge of MDR <em>A. baumannii</em>, emphasizing the need for vigilant surveillance and infection-control measures in healthcare settings in order to address these emerging threats effectively.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"38 \",\"pages\":\"Pages 327-331\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001395/pdfft?md5=c90865ae9f0839867ab7ef6c308f03b5&pid=1-s2.0-S2213716524001395-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001395\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001395","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Epidemiological pattern and genomic insights into multidrug-resistant ST491 Acinetobacter baumannii BD20 isolated from an infected wound in Bangladesh: Concerning co-occurrence of three classes of beta lactamase genes
Objective
Multidrug-resistant (MDR) Acinetobacter baumannii is a major issue within healthcare facilities in Bangladesh due to its frequent association with hospital-acquired infections. In this study we report on a carbapenem-resistant draft genome sequence of an A. baumannii BD20 sample isolated from an infected wound in Bangladesh.
Methods
A. baumannii BD20 was isolated from an infected burn wound. Whole-genome sequencing was carried out and annotated using PGAP and Prokka. Sequence type, antimicrobial resistance genes, virulence factor genes, and metal resistance genes were investigated. Core genome multilocus sequence typing–based phylogenomic analysis between A. baumannii BD20 and 213 A. baumannii strains retrieved from the NCBI GenBank database was performed using the BacWGSTdb 2.0 server.
Results
A. baumannii BD20 (MLST 491) was resistant to all the antibiotics tested, except for colistin and polymyxin B. Along with many other antibiotic resistance genes, the isolate harbored three classes of beta lactamase–producing genes: blaGES-11 (class A), blaOXA-69 (class D), blaADC-10 (class C), and blaADC-11 (class C). Additionally, the strain carried several virulence genes and metal resistance determinants, which may contribute to its increased virulence. Core genome MLST–based phylogenomic analysis revealed that A. baumannii BD20 was closely related to another ST491 strain isolated from Singapore.
Conclusions
The findings of this study underscore the growing challenge of MDR A. baumannii, emphasizing the need for vigilant surveillance and infection-control measures in healthcare settings in order to address these emerging threats effectively.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
