{"title":"酵母小类泛素修饰蛋白（SUMO）蛋白酶 Ulp2 参与了 RNA 剪接。","authors":"Jeong-Min Park, Seungji Choi, Dong Kyu Choi, Hyun-Shik Lee, Dong-Hyung Cho, Jungmin Choi, Hong-Yeoul Ryu","doi":"10.12717/DR.2024.28.2.47","DOIUrl":null,"url":null,"abstract":"<p><p>In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in <i>Saccharomyces cerevisiae</i>, the SUMO protease, Ulp2, is involved in splicing. In the <i>ulp2Δ</i> mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.</p>","PeriodicalId":72791,"journal":{"name":"Development & reproduction","volume":"28 2","pages":"47-54"},"PeriodicalIF":0.0000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268891/pdf/","citationCount":"0","resultStr":"{\"title\":\"Yeast Small Ubiquitin-Like Modifier (SUMO) Protease Ulp2 is Involved in RNA Splicing.\",\"authors\":\"Jeong-Min Park, Seungji Choi, Dong Kyu Choi, Hyun-Shik Lee, Dong-Hyung Cho, Jungmin Choi, Hong-Yeoul Ryu\",\"doi\":\"10.12717/DR.2024.28.2.47\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in <i>Saccharomyces cerevisiae</i>, the SUMO protease, Ulp2, is involved in splicing. In the <i>ulp2Δ</i> mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.</p>\",\"PeriodicalId\":72791,\"journal\":{\"name\":\"Development & reproduction\",\"volume\":\"28 2\",\"pages\":\"47-54\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11268891/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Development & reproduction\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12717/DR.2024.28.2.47\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/30 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Development & reproduction","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12717/DR.2024.28.2.47","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/30 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在真核生物中，RNA 剪接是一个重要的生物过程，对基因的精确表达至关重要。不准确的 RNA 剪接会导致 mRNA 生成异常，从而破坏蛋白质合成。据报道，为了调节剪接效率，一些剪接因子会发生类泛素修饰（SUMO）。我们的数据表明，在酿酒酵母中，SUMO蛋白酶Ulp2参与了剪接。在ulp2Δ突变体中，由于剪接不当，一些核糖体蛋白（RP）转录本中含有内含子的前mRNA水平显著增加。此外，我们还证实了 Ulp2 蛋白与 RP 基因内含子区的结合。这些发现凸显了Ulp2在RP转录本剪接中的关键作用。
Yeast Small Ubiquitin-Like Modifier (SUMO) Protease Ulp2 is Involved in RNA Splicing.
In eukaryotes, RNA splicing, an essential biological process, is crucial for precise gene expression. Inaccurate RNA splicing can cause aberrant mRNA production, disrupting protein synthesis. To regulate splicing efficiency, some splicing factors are reported to undergo Ubiquitin-like Modifier (SUMO)ylation. Our data indicate that in Saccharomyces cerevisiae, the SUMO protease, Ulp2, is involved in splicing. In the ulp2Δ mutant, some ribosomal protein (RP) transcripts exhibited a significant increase in the levels of intron-containing pre-mRNA because of improper splicing. Moreover, we confirmed Ulp2 protein binding to the intronic regions of RP genes. These findings highlight a critical Ulp2 role in RP transcript splicing.
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