使用培养的哺乳动物细胞进行衰老检测的简要指南。

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2024-07-23 DOI:10.1016/j.mocell.2024.100102

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引用次数: 0

摘要

细胞衰老是一个关键的生物学过程，与机体衰老和许多慢性疾病相关。在这里，我们将简要介绍哺乳动物衰老检测方法，包括细胞周期停滞、细胞形态变化、衰老相关β-半乳糖苷酶（SA-β-gal）染色和衰老相关分泌表型（SASP）表达的测量。这项工作将对具备细胞衰老测定最低专业知识的生物学家有所帮助。

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Brief guide to senescence assays using cultured mammalian cells

Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.