Khaya grandifoliola 茎皮乙醇提取物和水提取物对 Heligmosomoides polygyrus 的驱虫活性:体外和硅学方法。

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Journal of Tropical Medicine Pub Date : 2024-07-17 eCollection Date: 2024-01-01 DOI:10.1155/2024/6735764
Noumedem Anangmo Christelle Nadia, Mahdi Yaghoobi, Yamssi Cédric, Masoud Besati, Yungong Misparine Kiki, Ngouyamsa Nsapkain Aboubakar Sidiki, Mounvera Abdel Azizi, Vincent Khan Payne, Haibo Hu
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引用次数: 0

摘要

背景:寄生虫感染仍然是人类和牲畜的严重健康问题。本研究的目的是提供科学证据,证明卡亚树(Khaya grandifoliola)具有驱虫特性,当地人用它来治疗蠕虫病:方法:从实验感染小鼠的粪便样本中分离出新鲜的Heligmosomoides polygyrus虫卵。对粪便材料进行培养,分别在 48 小时和 120 小时后回收 L1 和 L2 幼虫。使用蠕虫微追踪器评估了提取物对多角体虫的驱虫效果。制备了两种不同的提取物(水提取物和乙醇提取物)。在进行杀卵和杀幼虫试验时,先将 100 µL 不同浓度的植物提取物、左旋咪唑和 1.5% 二甲基亚砜(DMSO)加入 96 孔微孔滴定板中,然后加入 100 µL 胚胎卵(60 个卵)进行杀卵试验,再加入 100 µL L 1 或 L 2 幼虫(50 只幼虫)进行杀幼虫试验。蠕虫在 27°C 的蠕虫微跟踪器中移动了 24 小时。使用 Schrodinger Maestro 软件的 Glide 模块进行对接研究:水提取物在 7.5 毫克/毫升时对孵化的抑制率最高,为 42.77 ± 12%。乙醇提取物(0.36 毫克/毫升)的 IC50 值表明,乙醇提取物对寄生虫从卵中孵化的能力有很好的抑制作用。在 7.5 mg/mL 的浓度下,乙醇提取物和水提取物对 L1 幼虫运动的抑制率分别为 98.0 ± 1.66% 和 83.33 ± 1.66%。阴性对照组蒸馏水和 1.5% DMSO 对幼虫没有抑制作用。对 L1 幼虫,首选药物左旋咪唑(阳性对照)的抑制率最高(100.0%)。六种化合物的对接得分最高,它们与受体的相互作用也最高。格兰酰胺 A 与酪氨酸、甘氨酸、苯丙氨酸、天冬酰胺和丝氨酸的相互作用最强,其苯环和氧原子对这些受体有抑制作用。羰基和羟基(OH)基团分别将格兰酰胺 D 与天冬酰胺、异亮氨酸和苯丙氨酸连接起来。通过 OH 基团向受体捐献氢,D-吡喃葡萄糖-6-磷酸与其氧结合磷和受体也形成相对较强的氢键。1-O-deacetylkhayanolide E 与丝氨酸和谷氨酸的相互作用最大。氨基甲酸的氨基甲酸苄酯[(1S)-1-苯基-2-[(4-甲基苯基)亚磺酰]乙基]与受体的羰基以及天冬酰胺和丝氨酸的相互作用最强。D-mannitol 含有丰富的氢氧化物,可作为氢供体和受体,与甘氨酸、天冬酰胺、天门冬氨酸、丙氨酸和谷氨酸等氨基酸的相互作用最为强烈:结论:K. grandifoliola 提取物具有驱虫特性。结论:大叶黄杨萃取物具有抗蠕虫特性,但仍需进行体内研究,以证明这种植物在治疗蠕虫病方面的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anthelmintic Activity of Ethanolic and Aqueous Extracts of Khaya grandifoliola Stem Bark against Heligmosomoides polygyrus: In Vitro and In Silico Approaches.

Background: Parasitic infection remains a serious health trade for humans and livestock. The purpose of this study was to present scientific proof of the anthelmintic properties of Khaya grandifoliola, which the native population uses to cure helminthiasis.

Method: Fresh Heligmosomoides polygyrus eggs were isolated from faecal samples of experimentally infected mice. The faecal material was cultured, and L1 and L2 larval stages were recovered after 48 and 120 hours, respectively. Using the worm microtracker, the anthelminthic efficacy of the extracts against H. polygyrus was assessed. Two different extracts (aqueous and ethanol extracts) were prepared. For the ovicidal and larvicidal activities, 100 µL of various concentrations of plant extracts, levamisole and 1.5% dimethyl sulfoxide (DMSO), were introduced into a 96-well microplate titer followed by the addition of 100 µL of embryonated eggs (60 eggs) for the ovicidal activity and 100 µL of L 1 or L 2 larvae (50 larvae) for the larvicidal activity. The movement of the worm was monitored for 24 hours in the worm microtracker at 27°C. The Glide module of the Schrodinger Maestro software was used to perform docking studies.

Results: For the aqueous extracts, the highest percentage of inhibition of hatching was 42.77 ± 12% at 7.5 mg/mL. The IC50 values for the ethanol (0.36 mg/mL) extract showed that the ethanol extract had a good inhibitory effect on the ability of parasites to hatch from eggs. The inhibition percentage of L1 larvae motility at 7.5 mg/mL was 98.0 ± 1.66% and 83.33 ± 1.66% for ethanol and aqueous extracts, respectively. The negative controls, distilled water and 1.5% DMSO, had no inhibitory impact on larvae. On L1-larvae, the drug of choice levamisole (positive control) had the highest percentage effect (100.0%). Six compounds had the highest docking score and their interactions with the receptor as well. Grandiamide A interacts most with tyrosine, glycine, phenylalanine, asparagine, and serine, and its benzene ring and oxygens inhibit these receptors. Carbonyl and hydroxyl (OH) groups connect grandiamide D to asparagine, isoleucine, and phenylalanine, respectively. By donating hydrogen to the receptor through OH groups, D-glucopyranose-6-phosphate also forms relatively strong hydrogen bonds with its oxygen-bound phosphorus and the receptor. 1-O-deacetylkhayanolide E interacts most with serine and glutamic acid. The carbamic acid benzyl ester of carbamic acid [(1S)-1-phenyl-2-[(4-methylphenyl) sulfinyl] ethyl] interacts most with the receptor with carbonyl groups and with asparagine and serine. With its abundant hydroxide, D-mannitol acts as a hydrogen donor and acceptor and interacts most strongly with amino acids such as glycine, asparagine, aspartic acid, alanine, and glutamic acid.

Conclusions: K. grandifoliola extracts possess anthelminthic properties. However, in vivo studies are still necessary to demonstrate the effectiveness of this plant for the treatment of helminthiasis.

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来源期刊
Journal of Tropical Medicine
Journal of Tropical Medicine Immunology and Microbiology-Parasitology
CiteScore
3.90
自引率
4.50%
发文量
0
审稿时长
14 weeks
期刊介绍: Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
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