{"title":"从油辣木叶中提取的黄酮类浓缩物可通过改善小鼠的能量代谢和抗氧化能力来延长其耗竭性游泳时间通过改善小鼠的能量代谢和抗氧化能力来延长竭尽全力游泳的时间","authors":"Xiangyu Bian, Lingling Wang, Yuying Ma, Yijing Yu, Changjiang Guo, Weina Gao","doi":"10.1089/jmf.2023.k.0114","DOIUrl":null,"url":null,"abstract":"<p><p><i>Moringa oleifera</i> Lam. leaves contain various nutrients and bioactive compounds. The present study aimed to assess the anti-fatigue capacity of a flavonoids concentrate purified from <i>M. oleifera</i> Lam. leaves. The total flavonoids in the purified extract were analyzed by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-MS/MS). The mice were supplemented with purified <i>M. oleifera</i> Lam. leaf flavonoid-rich extract (MLFE) for 14 days. The weight-loaded forced swimming test was used for evaluating exercise endurance. The 90-min non-weight-bearing swimming test was carried out to assess biochemical biomarkers correlated to fatigue and energy metabolism. UPLC-MS/MS analysis identified 83 flavonoids from MLFE. MLFE significantly increased the swimming time by 60%. Serum lactate (9.9 ± 0.9 vs. 8.9 ± 0.7), blood urea nitrogen (BUN) (8.8 ± 0.8 vs. 7.2 ± 0.5), and nonesterified fatty acid (NEFA) (2.4 ± 0.2 vs. 1.7 ± 0.3) were significantly elevated; <i>phosphoenolpyruvate carboxykinase (PEPCK)</i>, <i>glucokinase (GCK)</i>, and <i>nuclear factor erythroid 2-related factor 2 (Nrf2)</i> mRNA expression were significantly downregulated; and <i>heme oxygenase 1</i> mRNA expression was significantly upregulated in muscle after swimming. MLFE supplement significantly decreased serum lactate (8.0 ± 1.0 vs. 9.9 ± 0.9), BUN (8.6 ± 0.4 vs. 8.9 ± 0.8), and NEFA (2.3 ± 0.4 vs. 2.4 ± 0.2) and increased the protein and mRNA expression of <i>GCK</i>, <i>PEPCK</i>, and <i>Nrf2</i>. The enhancement of glucose metabolism and antioxidant function by MLFE contributes partly to its anti-fatigue action.</p>","PeriodicalId":16440,"journal":{"name":"Journal of medicinal food","volume":" ","pages":"887-894"},"PeriodicalIF":1.7000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Flavonoid Concentrate from <i>Moringa Oleifera</i> Lam. Leaves Extends Exhaustive Swimming Time by Improving Energy Metabolism and Antioxidant Capacity in Mice.\",\"authors\":\"Xiangyu Bian, Lingling Wang, Yuying Ma, Yijing Yu, Changjiang Guo, Weina Gao\",\"doi\":\"10.1089/jmf.2023.k.0114\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Moringa oleifera</i> Lam. leaves contain various nutrients and bioactive compounds. The present study aimed to assess the anti-fatigue capacity of a flavonoids concentrate purified from <i>M. oleifera</i> Lam. leaves. The total flavonoids in the purified extract were analyzed by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-MS/MS). The mice were supplemented with purified <i>M. oleifera</i> Lam. leaf flavonoid-rich extract (MLFE) for 14 days. The weight-loaded forced swimming test was used for evaluating exercise endurance. The 90-min non-weight-bearing swimming test was carried out to assess biochemical biomarkers correlated to fatigue and energy metabolism. UPLC-MS/MS analysis identified 83 flavonoids from MLFE. MLFE significantly increased the swimming time by 60%. Serum lactate (9.9 ± 0.9 vs. 8.9 ± 0.7), blood urea nitrogen (BUN) (8.8 ± 0.8 vs. 7.2 ± 0.5), and nonesterified fatty acid (NEFA) (2.4 ± 0.2 vs. 1.7 ± 0.3) were significantly elevated; <i>phosphoenolpyruvate carboxykinase (PEPCK)</i>, <i>glucokinase (GCK)</i>, and <i>nuclear factor erythroid 2-related factor 2 (Nrf2)</i> mRNA expression were significantly downregulated; and <i>heme oxygenase 1</i> mRNA expression was significantly upregulated in muscle after swimming. MLFE supplement significantly decreased serum lactate (8.0 ± 1.0 vs. 9.9 ± 0.9), BUN (8.6 ± 0.4 vs. 8.9 ± 0.8), and NEFA (2.3 ± 0.4 vs. 2.4 ± 0.2) and increased the protein and mRNA expression of <i>GCK</i>, <i>PEPCK</i>, and <i>Nrf2</i>. The enhancement of glucose metabolism and antioxidant function by MLFE contributes partly to its anti-fatigue action.</p>\",\"PeriodicalId\":16440,\"journal\":{\"name\":\"Journal of medicinal food\",\"volume\":\" \",\"pages\":\"887-894\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of medicinal food\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1089/jmf.2023.k.0114\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/7/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of medicinal food","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/jmf.2023.k.0114","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/7/25 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
摘要
油辣木叶含有多种营养物质和生物活性化合物。本研究旨在评估从油辣木叶中提纯的黄酮类浓缩物的抗疲劳能力。研究采用超高效液相色谱-电喷雾串联质谱法（UPLC-MS/MS）分析了纯化提取物中的总黄酮类化合物。小鼠连续 14 天服用纯化的油橄榄叶黄酮富集提取物（MLFE）。负重强迫游泳试验用于评估运动耐力。90 分钟无负重游泳测试用于评估与疲劳和能量代谢相关的生化生物标志物。UPLC-MS/MS分析从MLFE中鉴定出83种黄酮类化合物。MLFE可使游泳时间明显延长60%。血清乳酸（9.9 ± 0.9 vs. 8.9 ± 0.7）、血尿素氮（BUN）（8.8 ± 0.8 vs. 7.2 ± 0.5）和非酯化脂肪酸（NEFA）（2.4 ± 0.2 vs. 1.7 ± 0.3）明显升高；磷酸烯醇丙酮酸羧激酶（PEPCK）、葡萄糖激酶（GCK）和核因子红细胞 2 相关因子 2（Nrf2）mRNA 表达明显下调；游泳后肌肉中血红素加氧酶 1 mRNA 表达明显上调。补充 MLFE 能明显降低血清乳酸（8.0 ± 1.0 vs. 9.9 ± 0.9）、BUN（8.6 ± 0.4 vs. 8.9 ± 0.8）和 NEFA（2.3 ± 0.4 vs. 2.4 ± 0.2），并增加 GCK、PEPCK 和 Nrf2 的蛋白和 mRNA 表达。MLFE 可增强葡萄糖代谢和抗氧化功能，这是其抗疲劳作用的部分原因。
A Flavonoid Concentrate from Moringa Oleifera Lam. Leaves Extends Exhaustive Swimming Time by Improving Energy Metabolism and Antioxidant Capacity in Mice.
Moringa oleifera Lam. leaves contain various nutrients and bioactive compounds. The present study aimed to assess the anti-fatigue capacity of a flavonoids concentrate purified from M. oleifera Lam. leaves. The total flavonoids in the purified extract were analyzed by ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-MS/MS). The mice were supplemented with purified M. oleifera Lam. leaf flavonoid-rich extract (MLFE) for 14 days. The weight-loaded forced swimming test was used for evaluating exercise endurance. The 90-min non-weight-bearing swimming test was carried out to assess biochemical biomarkers correlated to fatigue and energy metabolism. UPLC-MS/MS analysis identified 83 flavonoids from MLFE. MLFE significantly increased the swimming time by 60%. Serum lactate (9.9 ± 0.9 vs. 8.9 ± 0.7), blood urea nitrogen (BUN) (8.8 ± 0.8 vs. 7.2 ± 0.5), and nonesterified fatty acid (NEFA) (2.4 ± 0.2 vs. 1.7 ± 0.3) were significantly elevated; phosphoenolpyruvate carboxykinase (PEPCK), glucokinase (GCK), and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA expression were significantly downregulated; and heme oxygenase 1 mRNA expression was significantly upregulated in muscle after swimming. MLFE supplement significantly decreased serum lactate (8.0 ± 1.0 vs. 9.9 ± 0.9), BUN (8.6 ± 0.4 vs. 8.9 ± 0.8), and NEFA (2.3 ± 0.4 vs. 2.4 ± 0.2) and increased the protein and mRNA expression of GCK, PEPCK, and Nrf2. The enhancement of glucose metabolism and antioxidant function by MLFE contributes partly to its anti-fatigue action.
期刊介绍：
Journal of Medicinal Food is the only peer-reviewed journal focusing exclusively on the medicinal value and biomedical effects of food materials. International in scope, the Journal advances the knowledge of the development of new food products and dietary supplements targeted at promoting health and the prevention and treatment of disease.
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