纳米姜黄素和巴豆素对骨髓间充质干细胞增殖和多能性的促进作用

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Nasim Sabouni, Mojgan Mohammadi, Amir Reza Boroumand, Sepideh Palizban, Jalil Tavakol Afshari
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引用次数: 0

摘要

目的:增强间充质干细胞的增殖、多能性和自我更新能力是其独有的特征,可提高其再生组织的治疗潜力。在这种情况下,具有突出药用特性的类胡萝卜素化合物黄腐素和姜黄素在细胞保护和生长方面大有可为。本研究旨在评估纳米姜黄素和巴豆素在体外对骨髓间充质干细胞增殖和多能性的影响:材料和方法:从干细胞治疗候选的 SCI 患者的髂嵴中分离出 BM-间充质干细胞。使用 MTT 和 PDT 试验评估了巴豆素和纳米姜黄素对间叶干细胞增殖的影响。流式细胞术测量了间充质干细胞凋亡的百分比。此外,实时 PCR 和 Western 印迹法分别定量检测了增殖和自我更新相关基因 OCT4 和 SOX2 的 mRNA 和蛋白表达:结果:我们的研究结果表明,只有低浓度的纳米姜黄素(0.3 和 0.7 µM)和巴豆素(2.5 5 µM)能显著影响间充质干细胞的增殖,并保护它们免于凋亡。此外,低剂量的巴豆素和纳米姜黄素还能提高 OCT4 和 SOX2 基因的 mRNA 和蛋白表达水平。相反,与未处理组相比,高浓度会降低间充质干细胞的存活率并导致细胞凋亡增加:我们的研究结果表明,在培养间充质干细胞时分别使用纳米姜黄素和巴豆素可被视为增殖剂,从而为细胞疗法准备更有利的工具。然而,这一领域还需要更多的体外和临床前研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Stimulating effect of nanocurcumin and crocin on proliferation and pluripotency of bone marrow-derived mesenchymal stem cells.

Objectives: Enhancement of proliferation, pluripotency, and self-renewal capacity as the unique features of MSCs can improve their therapeutic potential to regenerate tissues. In this context, crocin and curcumin, carotenoid compounds with outstanding medicinal properties, could be promising for cell protection and growth. This study aimed to evaluate the impact of nanocurcumin and crocin on BM-MSCs proliferation and pluripotency in vitro.

Materials and methods: BM-MSC were isolated from the iliac crest of SCI patients who were candidates for stem cell therapy. The effect of crocin and nanocurcumin on MSC proliferation was evaluated using MTT and PDT assay. The percentage of apoptotic MSCs was measured by flow cytometry. Furthermore, mRNA and protein expression of OCT4 and SOX2 as the proliferation and self-renewal related genes were quantified by real-time PCR and western blotting, respectively.

Results: Our findings demonstrated that only low concentrations of nanocurcumin (0.3 and 0.7 µM) and crocin (2.5 5 µM) significantly affected MSCs proliferation and protected them from apoptosis. Also, crocin and nanocurcumin at low doses caused an elevation in the mRNA and protein expression levels of OCT4 and SOX2 genes. In contrast, high concentrations decreased the survival of MSCs and led to increased apoptosis compared with the untreated group.

Conclusion: Our results suggest that using nanocurcumin and crocin separately in culturing MSCs can be considered proliferative agents to prepare the more advantageous tool for cell therapies. However, more in vitro and preclinical research is needed in this area.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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