营养性 microRNA:前体和成熟 microRNA 摄入后对目标基因的转录后调控以及木虱幼虫发育障碍。

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rutwik Bardapurkar, Gauri Binayak, Sagar Pandit
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引用次数: 0

摘要

微小RNA(miRNA)是转录后基因调控因子。在 miRNA 通路的细胞质部分,miRNA 从发夹结构的前体加工成双链(ds)成熟 RNA,最终加工成单链成熟 miRNA。在昆虫体内,摄取这两种ds形式的miRNA可以调节目标基因的表达;这启发了营养型miRNA作为功能基因组学和害虫管理工具的用途。然而,由于以往研究的侧重点不同,目前还没有系统的研究能够比较前体和成熟体、剂量、给药时间以及对目标转录本和表型的影响,这有助于开发一种 miRNA 给药方法。我们研究了经营养体输送的Px-let-7 miRNA对鳞翅目害虫木虱的影响,以比较其前期和ds成熟形式的功效。连续喂食添加了 miRNA 的食物抑制了目标蜕皮激素通路基因 FTZ-F1 和 E74 的表达。pre-let-7和成熟的let-7 miRNA形式同样下调了所有四龄幼虫的目标转录本。miRNA处理Dicer-1和AGO-1在摄入miRNA后上调,表明miRNA在幼虫组织中的系统扩散。乱序序列对照不影响目标转录本,表明成熟的 miRNA 具有序列特异性靶向作用,发夹盒不参与目标下调。这项工作为 miRNA 和靶基因功能分析提供了一个框架,并增强了营养型 miRNA 在害虫管理中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Trophic microRNA: Post-transcriptional regulation of target genes and larval development impairment in Plutella xylostella upon precursor and mature microRNA ingestion.

MicroRNAs (miRNAs) are post-transcriptional gene regulators. In the miRNA pathway's cytoplasmic part, the miRNA is processed from a hairpin-structured precursor to a double-stranded (ds) mature RNA and ultimately to a single-stranded mature miRNA. In insects, ingesting these two ds forms can regulate the target gene expression; this inspired the trophic miRNA's use as a functional genomics and pest management tool. However, systematic studies enabling comparisons of pre- and mature forms, dosages, administration times and instar-wise effects on target transcripts and phenotypes, which can help develop a miRNA administration method, are unavailable due to the different focuses of the previous investigations. We investigated the impact of trophically delivered Px-let-7 miRNA on the lepidopteran pest Plutella xylostella, to compare the efficacies of its pre- and ds-mature forms. Continuous feeding on the miRNA-supplemented diet suppressed expressions of FTZ-F1 and E74, the target ecdysone pathway genes. Both the pre-let-7 and mature let-7 miRNA forms similarly downregulated the target transcripts in all four larval instars. Pre-let-7 and let-7 ingestions decreased larval mass and instar duration and increased mortality in all instars, exhibiting adverse effects on larval growth and development. miRNA processing Dicer-1 and AGO-1's upregulations upon miRNA ingestion denoted the systemic miRNA spread in larval tissues. The scrambled sequence controls did not affect the target transcripts, suggesting the sequence-specific targeting by the mature miRNA and hairpin cassette's non-involvement in the target downregulation. This work provides a framework for miRNA and target gene function analyses and potentiates the trophic miRNA's utility in pest management.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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