{"title":"用于超灵敏检测癌胚抗原的双缺陷氮化石墨增强电化学发光。","authors":"Wenjie Chen , Xieyin Li , Minjia Tao , Hao Zhang , Qian Zhang , Yuchen Yue , Guifu Zuo , Junjian Zhao","doi":"10.1016/j.bioelechem.2024.108781","DOIUrl":null,"url":null,"abstract":"<div><p>Herein, a dual-defective graphite carbon nitride (DDCN) was prepared by polymerization under N<sub>2</sub> atmosphere combined with oxidation treatment. The luminous intensity of dual-defect graphite phase carbon nitride based on defect state luminescence is significantly improved compared to CN-air. On this basis, a biosensor for CEA detection was constructed based on specific immunobinding of antigen–antibody. It is noted that the biosensor exhibits a wide linear range of 1 × 10<sup>-5</sup> ∼ 1 × 10<sup>2</sup> ng•mL<sup>−1</sup>, a low detection limit of 3.3 × 10<sup>-4</sup> pg•mL<sup>−1</sup>, a recovery of 94 %∼105 % and RSD less than 4.41 %. In addition, there was no significant difference to the clinical results, indicating that this work has good clinical application prospects.</p></div>","PeriodicalId":252,"journal":{"name":"Bioelectrochemistry","volume":"160 ","pages":"Article 108781"},"PeriodicalIF":4.8000,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced electrochemiluminescence of dual-defect graphite carbon nitride for ultrasensitive detection of CEA\",\"authors\":\"Wenjie Chen , Xieyin Li , Minjia Tao , Hao Zhang , Qian Zhang , Yuchen Yue , Guifu Zuo , Junjian Zhao\",\"doi\":\"10.1016/j.bioelechem.2024.108781\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Herein, a dual-defective graphite carbon nitride (DDCN) was prepared by polymerization under N<sub>2</sub> atmosphere combined with oxidation treatment. The luminous intensity of dual-defect graphite phase carbon nitride based on defect state luminescence is significantly improved compared to CN-air. On this basis, a biosensor for CEA detection was constructed based on specific immunobinding of antigen–antibody. It is noted that the biosensor exhibits a wide linear range of 1 × 10<sup>-5</sup> ∼ 1 × 10<sup>2</sup> ng•mL<sup>−1</sup>, a low detection limit of 3.3 × 10<sup>-4</sup> pg•mL<sup>−1</sup>, a recovery of 94 %∼105 % and RSD less than 4.41 %. In addition, there was no significant difference to the clinical results, indicating that this work has good clinical application prospects.</p></div>\",\"PeriodicalId\":252,\"journal\":{\"name\":\"Bioelectrochemistry\",\"volume\":\"160 \",\"pages\":\"Article 108781\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-07-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioelectrochemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567539424001439\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectrochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567539424001439","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Enhanced electrochemiluminescence of dual-defect graphite carbon nitride for ultrasensitive detection of CEA
Herein, a dual-defective graphite carbon nitride (DDCN) was prepared by polymerization under N2 atmosphere combined with oxidation treatment. The luminous intensity of dual-defect graphite phase carbon nitride based on defect state luminescence is significantly improved compared to CN-air. On this basis, a biosensor for CEA detection was constructed based on specific immunobinding of antigen–antibody. It is noted that the biosensor exhibits a wide linear range of 1 × 10-5 ∼ 1 × 102 ng•mL−1, a low detection limit of 3.3 × 10-4 pg•mL−1, a recovery of 94 %∼105 % and RSD less than 4.41 %. In addition, there was no significant difference to the clinical results, indicating that this work has good clinical application prospects.
期刊介绍：
An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry
Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of:
• Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction.
• Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms)
• Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes)
• Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion)
• Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair).
• Organization and use of arrays in-vitro and in-vivo, including as part of feedback control.
• Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.
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