波罗的海咸水中菌落形成和单细胞微囊藻的氮获取策略和碳固定作用

IF 3.8 1区 地球科学 Q1 LIMNOLOGY
Christien P. Laber, Javier Alegria Zufia, Catherine Legrand, Elin Lindehoff, Hanna Farnelid
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引用次数: 0

摘要

微囊藻是一种广泛存在且对全球具有重要意义的初级生产者。在咸水中,微囊藻种群由多种物种组成,既有单细胞生活方式,也有集群生活方式。与海洋中的皮蓝藻相比,苦咸水中的皮蓝藻特征并不明显,研究重点也主要集中在单细胞皮蓝藻上。在此,我们利用 2020 年 8 月和 9 月期间波罗的海中部的碳-13 和无机(铵和硝酸盐)或有机(尿素和氨基酸)氮-15 双源培养箱,研究了单细胞和菌落型皮蓝藻的吸收动态。使用元素分析仪同位素比质谱仪(EA-IRMS)和纳米二次离子质谱仪(NanoSIMS)获得了浮游植物群落和特定群组的吸收率。在 9 月份,微囊藻对铵、尿素、氨基酸和无机碳群落吸收/固定的贡献率超过三分之一,但在浮游植物生物量较高的 8 月份,微囊藻的贡献率为 10%。总体而言,单细胞对铵和尿素的吸收率明显高于群落性微囊藻。在为期 6 年的波罗的海中部近海时间序列(2015-2020 年)中,夏季菌落状微囊藻的丰度高达 105 cells mL-1,占浮游植物平均生物量的 >5%,表明它们对生态系统具有周期性的重要作用。使用 16S rRNA 基因扩增片段数据无法识别藻群菌株,这表明需要改进工具来识别藻群形态。这项研究表明了单细胞咸水短笛藻对营养循环的重要性,以及在评估短笛藻在水生生态系统中的作用时考虑其吸收和生活策略的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Colony-forming and single-cell picocyanobacteria nitrogen acquisition strategies and carbon fixation in the brackish Baltic Sea

Colony-forming and single-cell picocyanobacteria nitrogen acquisition strategies and carbon fixation in the brackish Baltic Sea

Picocyanobacteria are widespread and globally significant primary producers. In brackish waters, picocyanobacterial populations are composed of diverse species with both single-cell and colony-forming lifestyles. Compared to their marine counterparts, brackish picocyanobacteria are less well characterized and the focus of research has been weighted toward single-cell picocyanobacteria. Here, we investigate the uptake dynamics of single and colony-forming picocyanobacteria using incubations with dual carbon-13 and inorganic (ammonium and nitrate) or organic (urea and amino acids) nitrogen-15 sources during August and September 2020 in the central Baltic Sea. Phytoplankton community and group-specific uptake rates were obtained using an elemental analyzer isotope ratio mass spectrometer (EA-IRMS) and nano secondary-ion mass spectrometry (NanoSIMS). Picocyanobacteria contributed greater than one third of the ammonium, urea, amino acids, and inorganic carbon community uptake/fixation in September but < 10% in August when phytoplankton biomass was higher. Overall, single-cell ammonium and urea uptake rates were significantly higher for single-celled compared to colonial picocyanobacteria. In a 6-yr offshore central Baltic Sea time series (2015–2020), summer abundances of colonial picocyanobacteria reached up to 105 cells mL−1 and represented > 5% of the average phytoplankton biomass, suggesting that they are periodically important for the ecosystem. Colonial strain identification was not distinguishable using 16S rRNA gene amplicon data, highlighting a need for refined tools for identification of colonial forms. This study shows the significance of single-celled brackish picocyanobacteria to nutrient cycling and the importance of considering uptake and lifestyle strategies when assessing the role of picocyanobacteria in aquatic ecosystems.

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来源期刊
Limnology and Oceanography
Limnology and Oceanography 地学-海洋学
CiteScore
8.80
自引率
6.70%
发文量
254
审稿时长
3 months
期刊介绍: Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.
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