用于检测体内肿瘤缺氧的对硝基还原酶敏感的近红外荧光生物传感器

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Safiya Nisar and Binglin Sui
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引用次数: 0

摘要

肿瘤细胞具有高代谢需求,导致耗氧量增加,进一步加剧了缺氧。缺氧一直被认为是实体瘤的一个特征,在肿瘤生长、抗药性和总体治疗效果方面起着重要作用。缺氧特异性传感探针目前急需为肿瘤检测和监测提供有价值的信息。在这项工作中,我们开发了一种具有高荧光量子产率的新型近红外荧光发射生物传感器,用于肿瘤组织中的缺氧检测。在肿瘤缺氧条件下,当硝基还原酶存在时,生物传感器分子中的硝基转化为氨基,由此产生的化合物通过自褪色过程变成无荧光染料,从而关闭了生物传感器的荧光发射。生物传感器对硝基还原酶的荧光变化具有灵敏性和选择性,不会受到其他重要生理物质的影响。在体外和体内生物成像实验中,该生物传感器在检测缺氧方面表现出很高的效率,并能区分不同大小的实体肿瘤,这表明它在肿瘤诊断和进展监测方面具有潜在的应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A nitroreductase-sensitive near-IR fluorescent biosensor for detecting tumor hypoxia in vivo†

A nitroreductase-sensitive near-IR fluorescent biosensor for detecting tumor hypoxia in vivo†

Tumor cells have high metabolic demands, leading to increased oxygen consumption and further exacerbating hypoxia, which has been regarded as a characteristic feature of solid tumors and plays a significant role in tumor growth, resistance to therapy, and overall treatment outcomes. Hypoxia-specific sensing probes are currently in urgent need to provide valuable information for tumor detection and monitoring. In this work, we developed a new near-IR fluorescence-emitting biosensor with a high fluorescence quantum yield for hypoxia detection in tumor tissues. In the presence of nitroreductase enzyme under tumor hypoxia, the nitro group of the biosensor molecule is converted into an amino group, and the resulting compound turns itself into a nonfluorescent dye through a self-immolating process, thus turning off the fluorescence emission of the biosensor. The fluorescence change of the biosensor in response to nitroreductase is sensitive and selective and is not influenced by the presence of other physiologically important species. In the in vitro and in vivo bioimaging experiments, the biosensor demonstrated high efficiency in detecting hypoxia and the capability of distinguishing solid tumors of different sizes, indicating its potential applications in tumor diagnosis and progression monitoring.

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CiteScore
2.30
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