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引用次数: 0
摘要
审查目的：过敏原提取物通常通过测量主要过敏原的浓度或通过高度过敏受试者与参考提取物的集合血清中 IgE 的竞争来测量 "整体过敏性"，从而对其进行标准化，以控制其效力。最近的发展为使用从高度过敏受试者身上克隆的人类 mAb 来确定过敏原提取物的效力提供了机会：最近的两项进展为修订过敏原提取物的效力测量提供了机会：从过敏受试者中克隆过敏原特异性 IgE 以及对主要过敏原蛋白进行广泛的表位图谱绘制。由于人类 IgE mAb 能识别与生物相关的表位，因此它们为确定过敏原提取物的效价提供了一个新的机会，并可能为过敏原标准化的科学基础做出贡献。
The Potential of Human Monoclonal IgE Antibodies to Establish Biological Potency and Stability of Allergen Extracts.
Purpose of review: Allergenic extracts are often standardized to control for potency, either by measuring concentrations of major allergens or "overall allergenicity" by competition for IgE in pooled sera from highly allergic subjects with a reference extract. Recent developments present an opportunity to use human mAb cloned from highly allergic subjects to define potency of allergenic extracts.
Recent findings: Two recent developments present an opportunity for revising potency measurements of allergen extracts: cloning allergen specific IgE from allergic subjects and extensive epitope mapping of major allergenic proteins. Because human IgE mAb recognize biologically relevant epitopes, they present a novel opportunity to determine the potencies of allergenic extracts and may contribute to the science base for allergen standardization.
期刊介绍：
The aim of Current Allergy and Asthma Reports is to systematically provide the views of highly selected experts on current advances in the fields of allergy and asthma and highlight the most important papers recently published. All reviews are intended to facilitate the understanding of new advances in science for better diagnosis, treatment, and prevention of allergy and asthma.
We accomplish this aim by appointing international experts in major subject areas across the discipline to review select topics emphasizing recent developments and highlighting important new papers and emerging concepts. We also provide commentaries from well-known figures in the field, and an Editorial Board of internationally diverse members suggests topics of special interest to their country/region and ensures that topics are current and include emerging research. Over a one- to two-year period, readers are updated on all the major advances in allergy and asthma.
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