系统性红斑狼疮患者体内显示低细胞毒性特征的循环 CD8 + LGALS9 + T 细胞群减少。

IF 3.3 4区 医学 Q3 IMMUNOLOGY
Immunologic Research Pub Date : 2024-12-01 Epub Date: 2024-07-24 DOI:10.1007/s12026-024-09522-4
Qi Li, Guochong Wang, Zihang Yuan, Rui Kang, Yaxin Li, Ayibaota Bahabayi, Ziqi Xiong, Zhonghui Zhang, Chen Liu
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引用次数: 0

摘要

LGALS9又称Galectin-9,是β-半乳糖苷酶家族的成员,在免疫调节中起着至关重要的作用。然而,它在 CD8 T 细胞中的表达和功能以及与细胞毒性 T 淋巴细胞(CTL)的关联仍不清楚。本研究旨在调查 LGALS9 在人体循环 CD8 T 淋巴细胞中的表达模式,并阐明其在系统性红斑狼疮(SLE)中的临床意义。研究人员采集了 56 名健康对照者和 50 名新发系统性红斑狼疮患者的血样。利用流式细胞术通过细胞内染色分析循环 CD8 T 淋巴细胞中 LGALS9 的表达。与 LGALS9 + CD8 + T 细胞相比,LGALS9-CD8 + T 细胞的颗粒酶 B (GZMB) 和穿孔素分泌增加,GPR56、CX3CR1、KLRD1、KLRF1、PD1 和 CD29 的表达水平升高。与 TEM(效应记忆 T 细胞)和 TEMRA(重新表达 CD45RA 的终末分化效应记忆 T 细胞)相比,Tn(幼稚 T 细胞)和 TCM(中枢记忆 T 细胞)显示 LGALS9 阳性的比例更高。在临床上,系统性红斑狼疮患者的 LGALS9 表达明显下调。LGALS9 + CD8 + T 细胞的曲线下面积(AUC)为 0.6916,而 LGALS9 + CD8 + T 细胞中的 CX3CR1 + 的曲线下面积(AUC)为 0.6478,KLRF1 + 的曲线下面积(AUC)为 0.6419,用于区分系统性红斑狼疮和健康人。总之,CD8 + LGALS9 + T细胞显示出低细胞毒性的特征,而且在系统性红斑狼疮患者中明显减少,这可能与系统性红斑狼疮的发病机制有关,并为诊断提供帮助。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Circulating CD8 + LGALS9 + T Cell Population Exhibiting Low Cytotoxic Characteristics are Decreased in Patients with Systemic Lupus Erythematosus.

Circulating CD8 + LGALS9 + T Cell Population Exhibiting Low Cytotoxic Characteristics are Decreased in Patients with Systemic Lupus Erythematosus.

LGALS9, also known as Galectin-9 and a member of the β-galactosidase family, plays a crucial role in immune regulation. However, its expression and function in CD8 T cells, as well as its association with cytotoxic T lymphocytes (CTL), remain unclear. This study aims to investigate LGALS9 expression patterns in human circulating CD8 T lymphocytes and elucidate its clinical significance in Systemic Lupus Erythematosus (SLE). Blood samples from 56 healthy controls and 50 new-onset SLE patients were collected. Flow cytometry was utilized to analyze LGALS9 expression in circulating CD8 T lymphocytes via intracellular staining. Compared to LGALS9 + CD8 + T cells, LGALS9-CD8 + T cells showed increased secretion of Granzyme B (GZMB) and Perforin, along with elevated expression levels of GPR56, CX3CR1, KLRD1, KLRF1, PD1, and CD29. A higher proportion of Tn (naive T cells) and TCM (central memory T cells) showed LGALS9 positivity, compared to TEM (effector memory T cells) and TEMRA (terminally differentiated effector memory T cells re-expressing CD45RA). Clinically, the downregulation of LGALS9 expression was significant in SLE patients. LGALS9 + CD8 + T cells exhibited an Area Under the Curve (AUC) of 0.6916, while CX3CR1 + in LGALS9 + CD8 + T cells had an AUC of 0.6478, and KLRF1 + had an AUC of 0.6419, for distinguishing SLE from healthy individuals. In conclusion, CD8 + LGALS9 + T cells display characteristics of low cytotoxicity, and their reduction is evident in SLE patients, potentially implicating them in SLE pathogenesis and providing diagnostic assistance.

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来源期刊
Immunologic Research
Immunologic Research 医学-免疫学
CiteScore
6.90
自引率
0.00%
发文量
83
审稿时长
6-12 weeks
期刊介绍: IMMUNOLOGIC RESEARCH represents a unique medium for the presentation, interpretation, and clarification of complex scientific data. Information is presented in the form of interpretive synthesis reviews, original research articles, symposia, editorials, and theoretical essays. The scope of coverage extends to cellular immunology, immunogenetics, molecular and structural immunology, immunoregulation and autoimmunity, immunopathology, tumor immunology, host defense and microbial immunity, including viral immunology, immunohematology, mucosal immunity, complement, transplantation immunology, clinical immunology, neuroimmunology, immunoendocrinology, immunotoxicology, translational immunology, and history of immunology.
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