评估卵巢反应不良患者卵泡液中硫醇/二硫化物的平衡。

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY
Esengul Türkyılmaz, Begün Erbaba, Salim Neşelioglu, Nafiye Karakaş Yılmaz, Özlem Moraloğlu Tekin
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引用次数: 0

摘要

研究目的本研究旨在比较接受卵胞浆内单精子显微注射(ICSI)的卵巢反应不良(POR)和卵巢反应正常(NOR)女性卵泡液(FF)中的硫醇/二硫化物平衡、髓过氧化物酶和缺血修饰白蛋白水平:研究于 2021 年 3 月至 2022 年 4 月在安卡拉市医院生殖医学中心妇产科进行。研究对象包括 27 名 POR 和 35 名 NOR 妇女,她们都接受了卵胞浆内单精子显微注射。FF是在控制性卵巢刺激周期后获得的。采用分光光度法检测 FF 的硫醇/二硫化物平衡。进行了相关分析,以确定这些氧化应激标记物是否有助于预测卵母细胞质量:结果:NOR 组的二硫化物水平明显高于 POR 组(P=0.014)。受精卵(2PN)数量与总硫醇水平呈正相关(r=0.258,p=0.046)。二硫化物水平与抗缪勒氏管激素水平(r=0.262,p=0.039)和取卵细胞总数(r=0.335,p=0.008)呈正相关:结论:NOR 组和 POR 组的二硫化物水平差异显著。结论:NOR 组和 POR 组的二硫化物水平差异显著,NOR 组和 POR 组的二硫化物水平差异显著,可能是由于 NOR 组的二硫化物水平升高所致。总硫醇水平与 2PN 卵母细胞总数相关。未来的研究应检查辅助生殖技术中心的硫醇/二硫化物平衡情况,以预测哪些卵母细胞可以受精。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the follicular fluid thiol/disulfide balance among patients with poor ovarian response.

Objective: This study aimed to compare the thiol/disulfide balance, myeloperoxidase, and ischemia-modified albumin levels in the follicular fluid (FF) of poor ovarian response (POR) and normal ovarian response (NOR) women who received intracytoplasmic sperm injection (ICSI).

Methods: The study was performed between March 2021 and April 2022 at the Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Ankara City Hospital. The study included 27 POR and 35 NOR women who underwent ICSI. FF was obtained after the controlled ovarian stimulation cycle. The FF thiol/disulfide balance was detected using spectrophotometric methods. A correlation analysis was conducted to determine whether these oxidative stress markers could contribute to predicting oocyte quality.

Results: Disulfide levels were significantly higher in the NOR group than in the POR group (p=0.014). The number of fertilized egg (2PN) oocytes was positively correlated with the total thiol level (r=0.258, p=0.046). The disulfide level was positively correlated with the anti-Müllerian hormone level (r=0.262, p=0.039) and the total number of retrieved oocytes (r=0.335, p=0.008).

Conclusion: The disulfide levels differed significantly between the NOR and POR groups. The statistically significant differences of fewer metaphase II oocytes and lower percentage of good-quality embryos in the NOR group compared to the POR group might have resulted from the NOR group's elevated disulfide levels. The total thiol levels correlated with the total of 2PN oocytes. Future studies should examine the thiol/disulfide balance at assisted reproductive technology centers to predict which oocytes could be fertilized.

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