Carolina dos Anjos, Yin Wang, Que Chi Truong-Bolduc, Paul K. Bolduc, Matthew Liu, David C. Hooper, R. Rox Anderson, Tianhong Dai, Leon G. Leanse
{"title":"蓝光削弱细菌 β-内酰胺酶的活性以克服 β-内酰胺抗药性","authors":"Carolina dos Anjos, Yin Wang, Que Chi Truong-Bolduc, Paul K. Bolduc, Matthew Liu, David C. Hooper, R. Rox Anderson, Tianhong Dai, Leon G. Leanse","doi":"10.1002/lsm.23819","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>In this study, we evaluated the effectiveness of antimicrobial blue light (aBL; 410 nm wavelength) against β-lactamase-carrying bacteria and the effect of aBL on the activity of β-lactamases.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p><i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, and <i>Klebsiella pneumoniae</i> strains carrying β-lactamases as well as a purified β-lactamase enzymes were studied. β-lactamase activity was assessed using a chromogenic cephalosporin hydrolysis assay. Additionally, we evaluated the role of porphyrins in the photoreaction, as well as protein degradation by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Finally, we investigated the bactericidal effect of combined aBL-ceftazidime exposure against a metallo-β-lactamase expressing <i>P. aeruginosa</i> strain<i>.</i></p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Our study demonstrated that aBL effectively killed β-lactamase-producing bacteria and reduced β-lactamase activity. After an aBL exposure of 1.52 J/cm<sup>2</sup>, a 50% reduction in enzymatic activity was observed in <i>P. aeruginosa</i>. Additionally, we found a 40% decrease in the photoreaction activity of porphyrins following an aBL exposure of 64.8 J/cm<sup>2</sup>. We also revealed that aBL reduced β-lactamase activity via protein degradation (after 136.4 J/cm<sup>2</sup>). Additionally, aBL markedly improved the bactericidal effect of ceftazidime (by >4-log<sub>10</sub>) in the metallo-β-lactamase <i>P. aeruginosa</i> strain.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Our results provide evidence that aBL compromises bacterial β-lactamase activity, offering a potential approach to overcome β-lactam resistance in bacteria.</p>\n </section>\n </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"56 7","pages":"673-681"},"PeriodicalIF":2.2000,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Blue Light Compromises Bacterial β-Lactamases Activity to Overcome β-Lactam Resistance\",\"authors\":\"Carolina dos Anjos, Yin Wang, Que Chi Truong-Bolduc, Paul K. Bolduc, Matthew Liu, David C. Hooper, R. Rox Anderson, Tianhong Dai, Leon G. Leanse\",\"doi\":\"10.1002/lsm.23819\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>In this study, we evaluated the effectiveness of antimicrobial blue light (aBL; 410 nm wavelength) against β-lactamase-carrying bacteria and the effect of aBL on the activity of β-lactamases.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p><i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, and <i>Klebsiella pneumoniae</i> strains carrying β-lactamases as well as a purified β-lactamase enzymes were studied. β-lactamase activity was assessed using a chromogenic cephalosporin hydrolysis assay. Additionally, we evaluated the role of porphyrins in the photoreaction, as well as protein degradation by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Finally, we investigated the bactericidal effect of combined aBL-ceftazidime exposure against a metallo-β-lactamase expressing <i>P. aeruginosa</i> strain<i>.</i></p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Our study demonstrated that aBL effectively killed β-lactamase-producing bacteria and reduced β-lactamase activity. After an aBL exposure of 1.52 J/cm<sup>2</sup>, a 50% reduction in enzymatic activity was observed in <i>P. aeruginosa</i>. Additionally, we found a 40% decrease in the photoreaction activity of porphyrins following an aBL exposure of 64.8 J/cm<sup>2</sup>. We also revealed that aBL reduced β-lactamase activity via protein degradation (after 136.4 J/cm<sup>2</sup>). 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Blue Light Compromises Bacterial β-Lactamases Activity to Overcome β-Lactam Resistance
Objective
In this study, we evaluated the effectiveness of antimicrobial blue light (aBL; 410 nm wavelength) against β-lactamase-carrying bacteria and the effect of aBL on the activity of β-lactamases.
Methods
Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae strains carrying β-lactamases as well as a purified β-lactamase enzymes were studied. β-lactamase activity was assessed using a chromogenic cephalosporin hydrolysis assay. Additionally, we evaluated the role of porphyrins in the photoreaction, as well as protein degradation by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Finally, we investigated the bactericidal effect of combined aBL-ceftazidime exposure against a metallo-β-lactamase expressing P. aeruginosa strain.
Results
Our study demonstrated that aBL effectively killed β-lactamase-producing bacteria and reduced β-lactamase activity. After an aBL exposure of 1.52 J/cm2, a 50% reduction in enzymatic activity was observed in P. aeruginosa. Additionally, we found a 40% decrease in the photoreaction activity of porphyrins following an aBL exposure of 64.8 J/cm2. We also revealed that aBL reduced β-lactamase activity via protein degradation (after 136.4 J/cm2). Additionally, aBL markedly improved the bactericidal effect of ceftazidime (by >4-log10) in the metallo-β-lactamase P. aeruginosa strain.
Conclusion
Our results provide evidence that aBL compromises bacterial β-lactamase activity, offering a potential approach to overcome β-lactam resistance in bacteria.
期刊介绍：
Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.
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