评估直接从阳性血培养物中提取的 Vitek-2 和 MicroScan AST：具有成本效益的 24 小时肠杆菌和葡萄球菌减毒法。

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2024-07-20 DOI:10.1016/j.mimet.2024.107003

M. Peradotto , L. Pangaro , C. Tavano , A. Ettori , A. Frigeri , E. Verri , G. Caffiero , L. Soattini , S. Daffara , L. Cianci , M. Pelagi

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引用次数: 0

摘要

缩短抗菌药物药敏试验（AST）的结果产生时间是一项核心需求，尤其是在败血症治疗方面。对于许多实验室来说，目前的自动快速 AST 仍然过于昂贵。我们的目的是评估三种前处理方法，以便在实验室现有的两种自动 AST 平台上实现当天接种。我们测试了 100 个肠杆菌或葡萄球菌阳性瓶。不同的方法和仪器都取得了良好的效果。特别是，当接种细菌颗粒时，Vitek-2 在肠杆菌 AST 方面表现出色（96.6% 的分类一致 - CA-，93.3% 的基本一致 - EA）。此外，葡萄球菌 AST 的短期培养菌落也有可接受的 CA（94.2%），尽管 EA 为 77.5%。MicroScan 系统对葡萄球菌 AST 的短期培养和直接血液接种的 CA 值均大于 95%，但 EA 值分别为 92.5%和 83.6%。另一方面，肠杆菌 AST 只有在接种细菌颗粒（CA 为 97.6%）时才显示出最佳性能。事实上，直接血液接种对几种分子显示出不可接受的参数。这两种系统都可以在快速、廉价的预处理后使用相同的常规活动仪器，从而缩短 24 小时内得出结果的时间。

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Evaluation of Vitek-2 and MicroScan ASTs directly from positive blood culture: A cost-effective 24-h reduction for Enterobacterales and staphylococci

The reduction of antimicrobial susceptibility testing (AST) time-to-result is a central need, especially in sepsis treatment. The current automated rapid ASTs are still too expensive for many laboratories.

We aimed to evaluate three pre-treatment methods for a same-day inoculation on both automated AST platforms available in our laboratory. We tested 100 Enterobacterales or staphylococci positive bottles.

We obtained good results with the different methods and instruments. In particular, Vitek-2 showed good performances with Enterobacterales AST when inoculated with bacterial pellet (96.6% categorical agreement - CA-, 93.3% essential agreement - EA). Also short-term incubation colonies for staphylococci AST had acceptable CA (94.2%), even if with 77.5% EA. MicroScan system for staphylococci AST with both short-term incubation and direct blood inoculation reached >95% CA, but 92.5% and 83.6% EA, respectively. On the other hand, Enterobacterales AST showed optimal performances only with bacterial pellet inoculation (97.6% CA). In fact, direct blood inoculation showed not acceptable parameters for several molecules.

Both systems allow a 24-h reduction in time-to-result, by using the same instruments of routine activity after rapid and cheap pre-treatments.

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.