MicroRNA-25抑制ADAMTS16导致肾细胞癌的致癌特性

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Jian Huang, Zhe Tang, Guangjun Zhong, Linjie Guo, Ye Feng
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引用次数: 0

摘要

导言:越来越多的证据表明,微RNA(miRNA)在调节肿瘤生长方面发挥着至关重要的作用。本研究主要探讨 miR-25 对肾细胞癌(RCC)进展的影响:研究人员检测了 miR-25 和 ADAMTS16 在 RCC 样本和细胞系中的表达水平。通过荧光素酶报告实验验证了 miR-25 和 ADAMTS16 之间的关联。利用 CCK-8 和流式细胞术评估了细胞活力、凋亡、迁移和侵袭,并通过 Western 印迹分析评估了 ADAMTS16、β-catenin、GSK-3β 和 p-GSK-3β 的表达水平:结果:研究发现,miR-25在RCC组织中的表达水平升高。结果:研究发现,miR-25在RCC组织中的表达水平升高,随后ADAMTS16被确定为miR-25的靶标。miR-25 水平的升高与 ADAMTS16 表达的降低有关,从而导致细胞活力增强、凋亡减少。相反,抑制 miR-25 则会降低细胞活力、增殖和迁移。此外,研究人员还观察到,miR-25 触发了 GSK-3β 和 β-catenin 的磷酸化,而 GSK-3β 的总水平不受影响:这项研究表明,miR-25通过Wnt/β-catenin信号通路调控ADAMTS16的表达,为RCC的病因和潜在治疗提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ADAMTS16 Suppression by MicroRNA-25 Leads to Oncogenic Properties in Renal Cell Carcinoma.

Introduction: Increasing evidence indicates that microRNAs (miRNAs) play a crucial role in modulating tumor growth. This study is centered on investigating the contribution of miR-25 to the progression of Renal Cell Carcinoma (RCC).

Methods: The investigators examined the expression levels of miR-25 and ADAMTS16 in RCC samples and cell lines. The association between miR-25 and ADAMTS16 was validated via a luciferase reporter assay. Cell viability, apoptosis, migration, and invasion were evaluated utilizing CCK-8 and flow cytometry techniques, while the expression levels of ADAMTS16, β-catenin, GSK-3β, and p-GSK-3β were assessed through western blot analysis.

Results: The investigation revealed elevated expression levels of miR-25 in RCC tissues. Subsequently, ADAMTS16 was identified as a target of miR-25. Increased miR-25 levels were associated with decreased expression of ADAMTS16, resulting in enhanced cell viability and diminished apoptosis. Conversely, inhibition of miR-25 led to decreased cell viability, proliferation, and migration. Additionally, the researchers observed that miR-25 triggered the phosphorylation of GSK-3β and β-catenin while leaving the total GSK-3β level unaffected.

Conclusion: This study suggests that miR-25 regulates the expression of ADAMTS16 through the Wnt/β-catenin signaling pathway, providing new insights into the cause and potential treatment of RCC.

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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
327
审稿时长
7.5 months
期刊介绍: Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.
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