克服大肠杆菌中异源蛋白表达量低或无表达的挑战的策略。

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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引用次数: 0

摘要

蛋白质表达是各种生物系统中的一个关键过程。大肠杆菌是工业催化和医疗保健领域广泛使用的微生物宿主,但研究人员在构建重组表达系统时经常面临重大挑战。要最大限度地发挥大肠杆菌表达系统的潜力,就必须解决某些目标蛋白质产量低或无产量的问题。本文针对大肠杆菌异源蛋白表达面临的主要挑战因素,包括蛋白毒性、基因序列的内在影响和 mRNA 结构,提出了可行的解决方案。这些策略包括管理毒性蛋白质表达的专门方法、解决与 mRNA 结构和密码子偏差相关的问题、考虑多种因素的先进密码子优化方法,以及由大数据和机器学习促进的新兴优化技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Strategies to overcome the challenges of low or no expression of heterologous proteins in Escherichia coli

Protein expression is a critical process in diverse biological systems. For Escherichia coli, a widely employed microbial host in industrial catalysis and healthcare, researchers often face significant challenges in constructing recombinant expression systems. To maximize the potential of E. coli expression systems, it is essential to address problems regarding the low or absent production of certain target proteins. This article presents viable solutions to the main factors posing challenges to heterologous protein expression in E. coli, which includes protein toxicity, the intrinsic influence of gene sequences, and mRNA structure. These strategies include specialized approaches for managing toxic protein expression, addressing issues related to mRNA structure and codon bias, advanced codon optimization methodologies that consider multiple factors, and emerging optimization techniques facilitated by big data and machine learning.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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