{"title":"[PM2.5 和 O3 亚慢性联合暴露对大鼠鼻黏膜 ATP 量和 ATP 酶活性的影响]。","authors":"Tenglong Yan, Jiayu Xu, Tian Chen, Xin Yang, Weiwei Wang, Shupei Zhou, Piye Niu, Guang Jia, Jiao Xia","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of fine particle matter (PM<sub>2.5</sub>) and ozone (O<sub>3</sub>) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats.</p><p><strong>Methods: </strong>Twenty male SD rats were divided into control group (<i>n</i>=10) and exposure group (<i>n</i>=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM<sub>2.5</sub> and O<sub>3</sub> in the exposure system were monitored, and a comprehensive assessment of PM<sub>2.5</sub> and O<sub>3</sub> in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na<sup>+</sup>-K<sup>+</sup> -ATPase and Ca<sup>2+</sup> -ATPase were detected by spectrophotometry. The <i>t</i> test of two independent samples was used to compare the differences among the indicator groups.</p><p><strong>Results: </strong>From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (<i>P</i> < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM<sub>2.5</sub> concentration in the exposure group was (30.68±19.23) μg/m<sup>3</sup>, and the maximum 8 h ozone concentration (O<sub>3</sub>-8 h) was (82.45±35.81) μg/m<sup>3</sup>. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na<sup>+</sup>-K<sup>+</sup>-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (<i>P</i> < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca<sup>2+</sup>-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups.</p><p><strong>Conclusion: </strong>The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM<sub>2.5</sub> and O<sub>3</sub>.</p>","PeriodicalId":8790,"journal":{"name":"北京大学学报（医学版）","volume":"56 4","pages":"687-692"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11284455/pdf/","citationCount":"0","resultStr":"{\"title\":\"[Effects of PM<sub>2.5</sub> and O<sub>3</sub> sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa].\",\"authors\":\"Tenglong Yan, Jiayu Xu, Tian Chen, Xin Yang, Weiwei Wang, Shupei Zhou, Piye Niu, Guang Jia, Jiao Xia\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To evaluate the effects of fine particle matter (PM<sub>2.5</sub>) and ozone (O<sub>3</sub>) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats.</p><p><strong>Methods: </strong>Twenty male SD rats were divided into control group (<i>n</i>=10) and exposure group (<i>n</i>=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM<sub>2.5</sub> and O<sub>3</sub> in the exposure system were monitored, and a comprehensive assessment of PM<sub>2.5</sub> and O<sub>3</sub> in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na<sup>+</sup>-K<sup>+</sup> -ATPase and Ca<sup>2+</sup> -ATPase were detected by spectrophotometry. The <i>t</i> test of two independent samples was used to compare the differences among the indicator groups.</p><p><strong>Results: </strong>From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (<i>P</i> < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM<sub>2.5</sub> concentration in the exposure group was (30.68±19.23) μg/m<sup>3</sup>, and the maximum 8 h ozone concentration (O<sub>3</sub>-8 h) was (82.45±35.81) μg/m<sup>3</sup>. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na<sup>+</sup>-K<sup>+</sup>-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (<i>P</i> < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca<sup>2+</sup>-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups.</p><p><strong>Conclusion: </strong>The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM<sub>2.5</sub> and O<sub>3</sub>.</p>\",\"PeriodicalId\":8790,\"journal\":{\"name\":\"北京大学学报（医学版）\",\"volume\":\"56 4\",\"pages\":\"687-692\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11284455/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"北京大学学报（医学版）\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"北京大学学报（医学版）","FirstCategoryId":"3","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
目的评估细颗粒物（PM2.5）和臭氧（O3）联合暴露对Sprague Dawley（SD）大鼠鼻黏膜三磷酸腺苷（ATP）量和ATP酶活性的影响：采用随机数字表法将 20 只雄性 SD 大鼠分为对照组（n=10）和暴露组（n=10）。暴露期间，监测暴露系统中PM2.5和O3的浓度，并结合自测和现场数据对暴露系统中的PM2.5和O3进行综合评估。在暴露 208 d 时，采集大鼠的核心、肝、脾、肾、睾丸等主要器官和鼻黏膜组织。称量每个器官的重量并计算器官系数。用生物发光法测定 ATP 的总量，用分光光度法检测 Na+-K+ -ATPase 和 Ca2+ -ATPase 的活性。采用两独立样本 t 检验比较各指标组间的差异：结果：从第3周开始至暴露期结束，暴露组大鼠体重高于对照组（P＜0.05），两组大鼠器官系数无显著差异。暴露组的 PM2.5 日平均浓度为（30.68±19.23）μg/m3，8 h 臭氧最大浓度（O3-8 h）为（82.45±35.81）μg/m3。暴露组大鼠鼻黏膜中 ATP 的化学发光值（792.4±274.1）IU/L 低于对照组（1 126.8±218.1）IU/L。暴露组大鼠鼻黏膜的 Na+-K+-ATP 酶活性（1.53±0.85）U/mg 低于对照组（4.31±1.60）U/mg（P < 0.05）。对照组和暴露组大鼠鼻黏膜蛋白质含量分别为（302.14±52.51）mg/L 和（234.58±53.49）mg/L，Ca2+-ATP 酶活性分别为（0.81±0.27）U/mg 和（0.99±0.73）U/mg。组间差异无学意义：结论：亚慢性低浓度暴露于PM2.5和O3条件下，大鼠鼻腔粘膜的动力能力下降。
[Effects of PM2.5 and O3 sub-chronic combined exposure on ATP amount and ATPase activities in rat nasal mucosa].
Objective: To evaluate the effects of fine particle matter (PM2.5) and ozone (O3) combined exposure on adenosine triphosphate (ATP) amount and ATPase activities in nasal mucosa of Sprague Dawley (SD) rats.
Methods: Twenty male SD rats were divided into control group (n=10) and exposure group (n=10) by random number table method. The rats were fed in the conventional clean environment and the air pollutant exposure system established by our team, respectively, and exposed for 208 d. During the exposure period, the concentrations of PM2.5 and O3 in the exposure system were monitored, and a comprehensive assessment of PM2.5 and O3 in the exposure system was conducted by combining self-measurement and site data. On the 208 d of exposure, the core, liver, spleen, kidney, testis and other major organs and nasal mucosal tissues of the rats were harvested. Each organ was weighed and the organ coefficient calculated. The total amount of ATP was measured by bioluminescence, and the activities of Na+-K+ -ATPase and Ca2+ -ATPase were detected by spectrophotometry. The t test of two independent samples was used to compare the differences among the indicator groups.
Results: From the 3rd week to the end of exposure duration, the body weight of the rats in the exposure group was higher than that in the control group (P < 0.05), and there was no significant difference in organ coefficients between the two groups. The average daily PM2.5 concentration in the exposure group was (30.68±19.23) μg/m3, and the maximum 8 h ozone concentration (O3-8 h) was (82.45±35.81) μg/m3. The chemiluminescence value (792.4±274.1) IU/L of ATP in nasal mucosa of the rats in the exposure group was lower than that in the control group (1 126.8±218.1) IU/L. The Na+-K+-ATPase activity (1.53±0.85) U/mg in nasal mucosa of the rats in the exposure group was lower than that in the control group (4.31±1.60) U/mg (P < 0.05). The protein content of nasal mucosa in the control group and the exposure group were (302.14±52.51) mg/L and (234.58±53.49) mg/L, respectively, and the activity of Ca2+-ATPase was (0.81±0.27) U/mg and (0.99±0.73) U/mg, respectively. There was no significant difference between the groups.
Conclusion: The ability of power capacity decreased in the rat nasal mucossa under the sub-chronic low-concentration exposure of PM2.5 and O3.
期刊介绍：
Beijing Da Xue Xue Bao Yi Xue Ban / Journal of Peking University (Health Sciences), established in 1959, is a national academic journal sponsored by Peking University, and its former name is Journal of Beijing Medical University. The coverage of the Journal includes basic medical sciences, clinical medicine, oral medicine, surgery, public health and epidemiology, pharmacology and pharmacy. Over the last few years, the Journal has published articles and reports covering major topics in the different special issues (e.g. research on disease genome, theory of drug withdrawal, mechanism and prevention of cardiovascular and cerebrovascular diseases, stomatology, orthopaedic, public health, urology and reproductive medicine). All the topics involve latest advances in medical sciences, hot topics in specific specialties, and prevention and treatment of major diseases.
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