大肠杆菌 ST131/O25b 的抗生素耐药性与生物膜形成的关系

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-07-22 Print Date: 2024-09-18 DOI:10.1556/030.2024.02275

Elif Aydın, Mustafa Kocaaga, Aybala Temel

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引用次数: 0

摘要

由于抗生素耐药性的存在，尿路感染每年都变得越来越难以治疗。尿路致病性大肠埃希菌（UPEC）分离株具有多重耐药性和生物膜形成的双重表现，构成了威胁。ST131 克隆是一种高风险的流行性克隆，因为它与抗菌药耐药性密切相关，近年来抗菌药耐药性的报道屡见不鲜。本研究旨在明确与 ST131/O25b UPEC 相关的风险因素、临床结果和细菌遗传学。本研究采用自动化系统对 61 株临床大肠杆菌进行了抗生素敏感性测定和菌种鉴定。通过双盘协同试验评估了扩谱β-内酰胺酶的检测情况。生物膜的形成采用分光光度法进行量化。通过聚合酶链式反应（PCR）研究了病毒基因（iutA、sfa cnf-1、iroN、afa、papA、fimA）、抗生素耐药基因（blaCTX-M、blaTEM、blaSHV、blaOXA、qnrA、qnrB、qnrS、ant（2'）-Ia、ant（3）-Ia、aac（3）-IIa、mcr-1、mcr-2、mcr-3、mcr-4）。发现了以下 beta-内酰胺酶基因：blaTEM（n = 53，86.8%）、blaCTX-M（n = 59，96.7%）、blaSHV（n = 47，77.0%）和 blaOXA-1（n = 27，44.2%）。我们的数据显示，93.4%（57/61）的大肠杆菌分离物是生物膜生产者。对 O25pabBspe 和 trpA2 进行了调查，以确定是否存在 ST131/O25b 克隆。在耐多药的分离株中，29 个分离株（47.5%）检测到 O25pabBspe 和 trpA2 共存。在 4 个具有较强生物膜产生能力的分离株中检测到了 fimH30 和 H30Rx 亚克隆。这些结果表明，临床大肠杆菌菌株可能成为毒力基因和抗生素耐药性基因的储存库。这项研究表明，大肠杆菌 ST131 和非 ST131 分离物在形成生物膜方面存在显著差异。此外，86.21%（n = 25）的 ST131 分离物能形成强至中度生物膜，而只有 43.75%（n = 14）的非 ST131 分离物能形成强生物膜。大多数 ST131 菌株中存在 iutA 和 fimA 基因，这表明它们在生物膜形成过程中发挥了重要作用。这些发现表明，iutA 和 fimA 基因抑制剂可用于治疗由产生生物膜的耐药 ST131 菌株引起的感染。

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Association of antibiotic resistance and biofilm formation in Escherichia coli ST131/O25b.

Urinary tract infections are becoming difficult to treat every year due to antibiotic resistance. Uropathogenic Escherichia coli (UPEC) isolates pose a threat with a combined expression of multidrug-resistance and biofilm formation. ST131 clone is a high-risk pandemic clone due to its strong association with antimicrobial resistance, which has been reported frequently in recent years. This study aims to define risk factors, clinical outcomes, and bacterial genetics associated with ST131/O25b UPEC. In this study, antibiotic susceptibility and species-level identification of 61 clinical E. coli strains were determined by automated systems. Detection of extended-spectrum beta-lactamases was assessed by double-disk synergy test. Biofilm formation was quantified by spectrophotometric method. Virulence genes (iutA, sfa cnf-1, iroN, afa, papA, fimA), antibiotic resistance genes (blaCTX-M, blaTEM, blaSHV, blaOXA, qnrA, qnrB, qnrS, ant(2')-Ia, ant(3)-Ia, aac(3)-IIa, mcr-1, mcr-2, mcr-3, mcr-4) were investigated by PCR. The following beta-lactamase genes were identified, blaTEM (n = 53, 86.8%), blaCTX-M (n = 59, 96.7%), blaSHV (n = 47, 77.0%), and blaOXA-1 (n = 27, 44.2%). Our data revealed that 93.4% of (57/61) E. coli isolates were biofilm-producers. O25pabBspe and trpA2 were investigated for the presence of ST131/O25b clone. Among multidrug resistant isolates, co-existence of O25pabBspe and trpA2 was detected in 29 isolates (47.5%). The fimH30 and H30Rx subclones were detected in four isolates that are strong biofilm-producers. These results suggest that clinical E. coli strains may become reservoirs of virulence and antibiotic resistance genes. This study demonstrates a significant difference in biofilm formation between E. coli ST131 and non-ST131 isolates. Moreover, 86.21% (n = 25) of ST131 isolates produced strong to moderate biofilms, while only 43.75% (n = 14) of non-ST131 isolates showed the ability to form strong biofilms. Presence of iutA and fimA genes in the majority of ST131 strains showed an important role in biofilm formation. These findings suggest application of iutA and fimA gene suppressors in treatment of infections caused by biofilm-producing drug-resistant ST131 strains.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.