利用粪肠球菌 DU.FS 衍生的外多糖生物合成抗菌和抗氧化银纳米粒子:绿色纳米技术的可持续方法

IF 4.7 3区 工程技术 Q2 ENGINEERING, ENVIRONMENTAL
Parvin Chegini, Fatemeh Salimi, Ehsan Nazarzadeh Zare, Parisa Farrokh
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引用次数: 0

摘要

纳米粒子的绿色合成是生产具有广谱生物活性的生物相容性纳米粒子的一种有效、经济、环保的方法。本研究探索了利用乳酸菌衍生的外多糖(EPS)来生物合成绿色银纳米粒子(EPS-AgNPs)。琼脂井扩散试验和 DPPH 试验分别考察了 EPS-AgNPs 的抗菌和抗氧化活性。随后,对 EPS-AgNPs 和化学 AgNPs 进行了表征和理化性质比较。根据 16 S rRNA 基因测序,产生 EPS 的乳酸菌与粪肠球菌的相似度为 97.77%。EPS-AgNPs 对革兰氏阳性和革兰氏阴性细菌病原体均有抑制作用。此外,EPS-AgNPs 还具有相当强的淬灭 DPPH 自由基的能力(84%)。傅立叶变换红外光谱(FT-IR)显示,EPS 的羟基、羰基和醛基等官能团参与了纳米粒子的合成。扫描电子显微镜显示,EPS-AgNPs 和化学合成的 AgNPs 均呈准球形形态,而 X 射线衍射(XRD)分析则证实了它们的结晶性质。化学 Ag-NPs 的金属含量高(89.79%),这与其 TGA 结果中的高热稳定性十分吻合。此外,EPS-AgNPs 较低的热稳定性和较少的银含量(65.93%)以及光滑的表面表明了 EPS 的封接作用。EPS-AgNPs 的 Z 平均尺寸、PI 值和 zeta 电位分别为 158.31 nm、0.29 和 - 17.75 mV。综上所述,本研究揭示了在绿色合成 EPS-AgNPs 的过程中,粪肠球菌衍生的 EPS 可作为还原剂、封端剂和稳定剂,有望应用于医疗和食品包装行业。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biogenic Synthesis of Antibacterial and Antioxidant Silver Nanoparticles Using Enterococcus faecium DU.FS-Derived Exopolysaccharides: A Sustainable Approach for Green Nanotechnology

Biogenic Synthesis of Antibacterial and Antioxidant Silver Nanoparticles Using Enterococcus faecium DU.FS-Derived Exopolysaccharides: A Sustainable Approach for Green Nanotechnology

Biogenic Synthesis of Antibacterial and Antioxidant Silver Nanoparticles Using Enterococcus faecium DU.FS-Derived Exopolysaccharides: A Sustainable Approach for Green Nanotechnology

Green synthesis of nanoparticles is an effective, cost-effective, and environmentally friendly approach to produce biocompatible nanoparticles with broad-spectrum bioactivities. In the current study, lactic acid bacterium-derived exopolysaccharide (EPS) was explored to biologically synthesize green silver nanoparticles (EPS-AgNPs). The antibacterial and antioxidant activities of EPS-AgNPs were investigated by agar well diffusion and DPPH tests, respectively. Subsequently, EPS-AgNPs and chemical AgNPs were characterized, and their physicochemical properties were compared. According to 16 S rRNA gene sequencing, the EPS-producing lactic acid bacterium showed 97.77% similarity to Enterococcus faecium. EPS-AgNPs exhibited inhibitory effects on Gram-positive and Gram-negative bacterial pathogens. Additionally, EPS-AgNPs showed considerable ability (84%) to quench DPPH free radicals. Fourier-transform infrared spectroscopy (FT-IR) showed the involvement of EPS’s functional groups, including hydroxyl, carbonyl, and aldehyde groups, in synthesizing nanoparticles. Scanning electron microscopy revealed a quasi-spherical morphology for both the EPS-AgNPs and chemically synthesized AgNPs, while X-ray diffraction (XRD) analysis confirmed their crystalline nature. The high metal content of chemical Ag-NPs (89.79%) was in good agreement with its high thermal stability in TGA results. Additionally, lower thermal stability and less Ag content (65.93%) of EPS-AgNPs, along with their smooth surfaces, indicated the capping role of EPS. EPS-AgNPs showed Z-average size, PI value, and zeta potential of 158.31 nm, 0.29, and − 17.75 mV, respectively. Collectively, the present study revealed that Enterococcus faecium-derived EPS acted as reducing, capping, and stabilizing agents in green synthesizing EPS-AgNPs with potential applications in medical and food-packaging industries.

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来源期刊
Journal of Polymers and the Environment
Journal of Polymers and the Environment 工程技术-高分子科学
CiteScore
9.50
自引率
7.50%
发文量
297
审稿时长
9 months
期刊介绍: The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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