THP-1 巨噬细胞在模型感染中限制中性粒细胞跨内皮迁移

IF 2.3 4区 医学 Q3 BIOPHYSICS
Aitana Ignes-Romeu, Hannah K. Weppner, Tanisha Kaur, Maya Singh, Laurel E. Hind
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引用次数: 0

摘要

导言中性粒细胞功能失调在感染、癌症、心血管疾病和自身免疫性疾病的病理过程中起着重要作用。中性粒细胞的活性受多种细胞群的影响,其中巨噬细胞是关键的调节因子。我们对 "芯片感染 "微流控装置进行了改装,将巨噬细胞整合到胶原细胞外基质中,从而可以在模拟体内环境的条件下研究人中性粒细胞和巨噬细胞之间的相互作用。对 THP-1 巨噬细胞的整合进行了优化,并描述了它们对内皮腔的影响,重点是通透性和结构完整性。然后利用该装置检测了巨噬细胞对中性粒细胞感染细菌病原体铜绿假单胞菌反应的影响。结果在微流控装置中成功优化了 THP-1 巨噬细胞的整合,结果显示内皮通透性和结构损伤没有增加。结论我们的研究结果突显了巨噬细胞在调节中性粒细胞反应中的调控作用,为控制中性粒细胞在各种疾病中的功能提出了潜在的治疗靶点。改良的微流控平台为研究疾病中巨噬细胞与中性粒细胞相互作用的机理提供了宝贵的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

THP-1 Macrophages Limit Neutrophil Transendothelial Migration in a Model Infection

THP-1 Macrophages Limit Neutrophil Transendothelial Migration in a Model Infection

Introduction

Dysregulated neutrophil function plays a significant role in the pathology of infections, cancer, cardiovascular diseases, and autoimmune disorders. Neutrophil activity is influenced by various cell populations, including macrophages, which are crucial regulators. However, the exact role of human macrophages in controlling neutrophil function remains unclear due to a scarcity of studies utilizing human cells in physiologically relevant models.

Methods

We adapted our “Infection-on-a-Chip” microfluidic device to incorporate macrophages within the collagen extracellular matrix, allowing for the study of interactions between human neutrophils and macrophages in a context that mimics in vivo conditions. The integration of THP-1 macrophages was optimized and their effect on the endothelial lumen was characterized, focusing on permeability and structural integrity. The device was then employed to examine the influence of macrophages on neutrophil response to infection with the bacterial pathogen Pseudomonas aeruginosa.

Results

Integration of THP-1 macrophages into the microfluidic device was successfully optimized, showing no increase in endothelial permeability or structural damage. The presence of macrophages was found to significantly reduce neutrophil transendothelial migration in response to Pseudomonas aeruginosa infection.

Conclusions

Our findings highlight the regulatory role of macrophages in modulating neutrophil responses, suggesting potential therapeutic targets to control neutrophil function in various diseases. The modified microfluidic platform offers a valuable tool for mechanistic studies into macrophage-neutrophil interactions in disease contexts.

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来源期刊
CiteScore
5.60
自引率
3.60%
发文量
30
审稿时长
>12 weeks
期刊介绍: The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.
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