使用双功能碳点掺杂分子印迹聚合物检测和吸附牛奶中的氟苯尼考。

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Weiyan Li, Chuansheng Sun, Haiping Wang, Qingyan Bai, Yi Xu, Chunmiao Bo, Junjie Ou
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引用次数: 0

摘要

作为最广泛使用的抗生素之一,检测动物源食品中氟苯尼考(FF)的残留量对食品安全至关重要。利用表面引发原子转移自由基聚合技术,以聚(甲基丙烯酸缩水甘油酯-甲基丙烯酸乙二醇酯)微球、4-乙烯基吡啶、甲基丙烯酸乙二醇酯和氟苯尼考为基质、功能单体、交联剂和模板分子,合成了荧光分子印迹聚合物(MIP)。同时,以柠檬酸三铵和硫脲为前驱体,在 400 W 的微波辐照下 2.5 分钟合成了 N-S 共掺杂碳点(CD),然后将其整合到 FF-MIP 中,得到 CD@FF-MIP。为了进行比较,还制备了不含 FF 的非压印聚合物(NIP)。CD@FF-MIP 对 FF 的吸附容量达到 53.1 mg g-1,高于 FF-MIP 的吸附容量(34.7 mg g-1),而 NIP 的吸附容量仅为 17.3 mg g-1。三种材料在 50 分钟内达到了吸附平衡。特别是,在 3-50 µmol L-1 的浓度范围内,CD@FF-MIP 对 FF 具有极好的荧光淬灭响应。因此,CD@FF-MIP 被成功用于提取牛奶样品中的 FF,并通过高效液相色谱法进行分析。标准回收率为95.8%-98.2%,相对标准偏差为1.6%-4.2%。该方法具有操作简单、灵敏度高、选择性好、成本低等优点,在食品检测中具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection and adsorption of florfenicol in milk using bifunctional carbon dot-doped molecularly imprinted polymers.

Detection of florfenicol (FF) residues in animal-derived foods, as one of the most widely used antibiotics, is critically important to food safety. The fluorescent molecularly imprinted polymer (MIP) was synthesized by surface-initiated atom transfer radical polymerization technique with poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) microspheres, 4-vinylpyridine, ethylene glycol dimethacrylate, and FF as the matrix, functional monomer, crosslinker, and template molecule, respectively. Meanwhile, N-S co-doped carbon dot (CD) was synthesized with triammonium citrate and thiourea as precursors under microwave irradiation at 400 W for 2.5 min and then integrated into FF-MIP to obtain CD@FF-MIP. For comparison, non-imprinted polymer (NIP) without FF was also prepared. The adsorption capacity of CD@FF-MIP to FF reached 53.1 mg g-1, which was higher than that of FF-MIP (34.7 mg g-1), whereas the adsorption capacity of NIP was only 17.3 mg g-1. The adsorption equilibrium of three materials was reached within 50 min. Particularly, CD@FF-MIP exhibited an excellent fluorescence quenching response to FF in the concentration range of 3-50 µmol L-1. As a result, CD@FF-MIP was successfully utilized to extract FF in milk samples, which were analyzed by high-performance liquid chromatography. The standard recoveries were 95.8%-98.2%, and the relative standard deviation was 1.6%-4.2%. The method showed the advantages of simple operation, high sensitivity, excellent selectivity, and low cost, and also demonstrated a great application prospect in food detection.

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来源期刊
ELECTROPHORESIS
ELECTROPHORESIS 生物-分析化学
CiteScore
6.30
自引率
13.80%
发文量
244
审稿时长
1.9 months
期刊介绍: ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.
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