Tingting Pan , Jing Zhang , Che Hu , Guanxiong Yan , Weiwei Qin , Zixin Cheng , Dongxia Yuan , Fang Zhou , Jie Xiong , Wei Miao , Chuanqi Jiang
{"title":"纤毛真核生物管蛋白染色的改进方法","authors":"Tingting Pan , Jing Zhang , Che Hu , Guanxiong Yan , Weiwei Qin , Zixin Cheng , Dongxia Yuan , Fang Zhou , Jie Xiong , Wei Miao , Chuanqi Jiang","doi":"10.1016/j.watbs.2024.100274","DOIUrl":null,"url":null,"abstract":"<div><p>Ciliated microeukaryotes (ciliates) are distinguished by their cilia, which are rich in tubulin. We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process, which is effective across diverse ciliate lineages. Moreover, our method allows integration with immunofluorescence staining using antibodies when needed. The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.</p></div>","PeriodicalId":101277,"journal":{"name":"Water Biology and Security","volume":"3 3","pages":"Article 100274"},"PeriodicalIF":5.1000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772735124000350/pdfft?md5=84f89319fcf12dd9cfbe56c612904f77&pid=1-s2.0-S2772735124000350-main.pdf","citationCount":"0","resultStr":"{\"title\":\"An improved method for tubulin staining of ciliated eukaryotes\",\"authors\":\"Tingting Pan , Jing Zhang , Che Hu , Guanxiong Yan , Weiwei Qin , Zixin Cheng , Dongxia Yuan , Fang Zhou , Jie Xiong , Wei Miao , Chuanqi Jiang\",\"doi\":\"10.1016/j.watbs.2024.100274\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ciliated microeukaryotes (ciliates) are distinguished by their cilia, which are rich in tubulin. We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process, which is effective across diverse ciliate lineages. Moreover, our method allows integration with immunofluorescence staining using antibodies when needed. The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.</p></div>\",\"PeriodicalId\":101277,\"journal\":{\"name\":\"Water Biology and Security\",\"volume\":\"3 3\",\"pages\":\"Article 100274\"},\"PeriodicalIF\":5.1000,\"publicationDate\":\"2024-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2772735124000350/pdfft?md5=84f89319fcf12dd9cfbe56c612904f77&pid=1-s2.0-S2772735124000350-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water Biology and Security\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772735124000350\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Biology and Security","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772735124000350","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
An improved method for tubulin staining of ciliated eukaryotes
Ciliated microeukaryotes (ciliates) are distinguished by their cilia, which are rich in tubulin. We developed a method for tubulin staining in ciliate cells that involves using live-cell tubulin-staining dyes instead of antibodies thereby streamlining the staining process, which is effective across diverse ciliate lineages. Moreover, our method allows integration with immunofluorescence staining using antibodies when needed. The potential applications of this technique extend to cell biology and ciliate morphological and ecological studies.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
