揭开副颈形成和功能的神秘面纱

IF 6 2区 生物学 Q1 CELL BIOLOGY
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引用次数: 0

摘要

当 NEAT1 长非编码 RNA 转录并被多个 RNA 结合蛋白结合时,就会形成副颈核体。20年前,人们首次描述了副斑块,近年来,人们对副斑块动力学的认识不断加深,从而对其结构和功能都有了新的理解。从结构上看,副颈通过不同的物理化学结构域形成,这些结构域由关键蛋白组成,并被招募到 NEAT1 的不同部分。这些结构域相互作用,通过微相分离形成核壳结构的副斑块。在功能上,许多环境、化学和机械触发因素都能改变副壳的丰度,其重要后果取决于细胞类型、发育阶段和触发因素特性。这些见解的基础是副壁细胞研究的新工具,包括筛选测定法、基于邻近性的识别工具和 RNA 处理调节剂。在许多健康和疾病环境中,副颈作为基因调控凝聚体的图景正在出现。然而,至关重要的是,当细胞和生物体面临外部压力时,副斑块的功能重要性通常最为明显。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling the intricacies of paraspeckle formation and function

Unveiling the intricacies of paraspeckle formation and function

Paraspeckle nuclear bodies form when the NEAT1 long noncoding RNA is transcribed and bound by multiple RNA-binding proteins. First described 20 years ago, in recent years a growing appreciation of paraspeckle dynamics has led to new understandings, in both structure and function. Structurally, paraspeckles form via distinct physico-chemical domains arising from the composition of key proteins, recruited to different parts of NEAT1. These domains interact, creating a core–shell structured paraspeckle via microphase separation. Functionally, many environmental, chemical, and mechanical triggers can alter paraspeckle abundance, with important consequences depending on the cell type, developmental stage, and trigger identity. Underpinning these insights are new tools for paraspeckle research, including screening assays, proximity-based identification tools, and RNA processing modulators. A picture is emerging of paraspeckles as gene regulatory condensates in many healthy and disease settings. Critically, however, paraspeckle functional importance is generally most apparent when cells and organisms face external stressors.

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来源期刊
Current Opinion in Cell Biology
Current Opinion in Cell Biology 生物-细胞生物学
CiteScore
14.60
自引率
1.30%
发文量
79
审稿时长
93 days
期刊介绍: Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.
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