使用分光荧光仪评估柠檬酸和乙二胺四乙酸对附着在模拟脱落恒牙上的培养人牙周韧带细胞活力的影响--一项体外研究。

IF 2.3 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Avani Ramesh Doiphode, Ritesh Rambharos Kalaskar
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引用次数: 0

摘要

背景/目的:延迟重新种植脱落的牙齿会导致牙根强直,随后发生替代吸收,最终在 2-4 年内牙齿脱落。为防止牙齿脱落,可以用酸腐蚀牙根表面,以暴露牙骨质层中的胶原纤维。这一过程有利于牙周韧带的正常重新附着和再生。这项体外研究旨在评估经柠檬酸和乙二胺四乙酸(EDTA)溶液处理的脱水模拟脱落牙根表面上附着的培养人牙周韧带细胞(HPLC)的活力和数量:研究对象为健全的人类恒牙。将牙齿根部切片,风干 1 小时后分为以下三组:A 组--对照组;B 组--柠檬酸处理 30 分钟组;C 组--EDTA 处理 5 分钟组。然后将切片放入培养的 HPLC 中。培养 24 小时后,在显微镜下观察切片,并准备使用分光荧光计读取存活和死亡的 HPLC,以及在 Neubauer 室中对 HPLC 进行计数:分光荧光计显示的存活和死亡 HPLC 强度差异有统计学意义(p = .003 和 p = .002),柠檬酸组的平均存活 HPLC 强度更高(69.52 ± 74.51),其次是 EDTA 组(31.39 ± 9.12)和对照组(-130.93 ± 30.99)。乙二胺四乙酸组(19.43 ± 47.31)、柠檬酸组(1.28 ± 1.85)和对照组(-2.77 ± 0.76)的高效液相色谱死细胞数较多。Neubauer 室中的细胞总数差异有统计学意义(p 结论:柠檬酸组和对照组的细胞总数差异无统计学意义(p 结论:柠檬酸组和对照组的细胞总数差异有统计学意义(p):与 EDTA 治疗 5 分钟组和对照组相比,在脱水牙根表面使用柠檬酸 30 分钟具有更好的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of the effects of citric acid and EDTA on cell viability of cultured human periodontal ligament cells attached to simulated avulsed permanent tooth using a spectrofluorometer-An in vitro study.

Background/aim: The delayed re-implantation of avulsed teeth results in ankylosis, followed by replacement resorption and eventual loss of the tooth within 2-4 years. To prevent tooth loss, the root surface can be etched with acid to expose the collagen fibers present in the cementum layer. This process facilitates normal reattachment and regeneration of the periodontal ligament. This in-vitro study aimed to assess the viability and number of attached cultured Human Periodontal Ligament Cells (HPLC) on the dehydrated root surface of simulated avulsed teeth treated with citric acid and EDTA solutions.

Materials and methods: Sound human permanent teeth were included in the study. The root portions of the teeth were sectioned into slices, air-dried for 1 h, and divided into the following three groups: Group A-control; Group B-Citric acid treated for 30 min; Group C-EDTA treated for 5 min. The slices were then placed in cultured HPLC. After a 24-h incubation period, the slices were visualized under the microscope and prepared for reading the viable and dead HPLC using a spectrofluorometer, as well as for counting HPLC in a Neubauer Chamber.

Results: The spectrofluorometer intensity for viable and dead HPLC showed a statistically significant difference (p = .003 and p = .002), with the mean intensity for viable HPLC greater in citric acid group (69.52 ± 74.51), followed by EDTA group (31.39 ± 9.12), and control group (-130.93 ± 30.99). The dead HPLC intensity was greater in the EDTA group (19.43 ± 47.31), followed by the citric acid group (1.28 ± 1.85), and the control group (-2.77 ± 0.76). The total number of cells in the Neubauer chamber showed a statistically significant difference (p < 0.001), with a higher count in the citric acid group (10.83 ± 4.08) followed by EDTA group (2.92 ± 2.92).

Conclusion: The application of citric acid for 30 min on the dehydrated root surface of avulsed teeth demonstrated superior outcomes compared to both EDTA treatment for 5 min and the control group.

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来源期刊
Dental Traumatology
Dental Traumatology 医学-牙科与口腔外科
CiteScore
6.40
自引率
32.00%
发文量
85
审稿时长
6-12 weeks
期刊介绍: Dental Traumatology is an international journal that aims to convey scientific and clinical progress in all areas related to adult and pediatric dental traumatology. This includes the following topics: - Epidemiology, Social Aspects, Education, Diagnostics - Esthetics / Prosthetics/ Restorative - Evidence Based Traumatology & Study Design - Oral & Maxillofacial Surgery/Transplant/Implant - Pediatrics and Orthodontics - Prevention and Sports Dentistry - Endodontics and Periodontal Aspects The journal"s aim is to promote communication among clinicians, educators, researchers, and others interested in the field of dental traumatology.
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