{"title":"利用假单胞菌(Pseudomonas putida)和节杆菌(Arthrobacter sp:性能与机理","authors":"","doi":"10.1016/j.bej.2024.109433","DOIUrl":null,"url":null,"abstract":"<div><p>The strain species resource is essential for microbial remediation of PHE (phenanthrene)-Cd<sup>2+</sup> co-contamination. In this study, the mixed bacterial culture (M) was established to intensively remediate PHE-Cd<sup>2+</sup> co-contamination using PAHs-degrading bacteria <em>Pseudomonas putida</em> and <em>Arthrobacter</em> sp. with different Cd<sup>2+</sup> tolerance. The PHE degradation efficiency of the mixed bacterial cultures (M) was 65 %-81 % under different Cd<sup>2+</sup> concentration of 10–50 mg/L, respectively, which was 1.40–2.98 times that of the individual strains. In addition, strain metabolic enzyme activity, intracellular structure and extracellular polymeric substances (EPS) were carried out under Cd<sup>2+</sup> stress. Results showed that the catechol 1,2-dioxygenase enzyme (C120) and the electron transport system activity (ETSA) were less adversely affected by Cd<sup>2+</sup> in mixed bacterial cultures. Transmission electron microscopy images showed that the cells surface of the mixed bacterial cultures (M) could be adsorbed more Cd<sup>2+</sup> compared to the single strain. The analysis of cell functional groups suggested that C-O-C and C-O groups in EPS mediated the removal of Cd<sup>2+</sup> in the mixed bacterial culture. Moreover, the Cd<sup>2+</sup> removal proportion by EPS adsorption of the mixed bacterial cultures accounted for 92 %, 67 %, 57 %, and 52 % under Cd<sup>2+</sup> stress of 0.5, 10, 25, and 50 mg/L, respectively, which were superior to that of individual strains. This study confirmed the potential application and technical reference of the specific mixed bacterial cultures in the enhanced bioremediation of PHE-Cd<sup>2+</sup> co-contamination.</p></div>","PeriodicalId":8766,"journal":{"name":"Biochemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhanced removal of PHE-Cd2+ co-contamination by the mixed bacterial cultures of Pseudomonas putida and Arthrobacter sp.: Performance and mechanism\",\"authors\":\"\",\"doi\":\"10.1016/j.bej.2024.109433\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The strain species resource is essential for microbial remediation of PHE (phenanthrene)-Cd<sup>2+</sup> co-contamination. In this study, the mixed bacterial culture (M) was established to intensively remediate PHE-Cd<sup>2+</sup> co-contamination using PAHs-degrading bacteria <em>Pseudomonas putida</em> and <em>Arthrobacter</em> sp. with different Cd<sup>2+</sup> tolerance. The PHE degradation efficiency of the mixed bacterial cultures (M) was 65 %-81 % under different Cd<sup>2+</sup> concentration of 10–50 mg/L, respectively, which was 1.40–2.98 times that of the individual strains. In addition, strain metabolic enzyme activity, intracellular structure and extracellular polymeric substances (EPS) were carried out under Cd<sup>2+</sup> stress. Results showed that the catechol 1,2-dioxygenase enzyme (C120) and the electron transport system activity (ETSA) were less adversely affected by Cd<sup>2+</sup> in mixed bacterial cultures. Transmission electron microscopy images showed that the cells surface of the mixed bacterial cultures (M) could be adsorbed more Cd<sup>2+</sup> compared to the single strain. The analysis of cell functional groups suggested that C-O-C and C-O groups in EPS mediated the removal of Cd<sup>2+</sup> in the mixed bacterial culture. Moreover, the Cd<sup>2+</sup> removal proportion by EPS adsorption of the mixed bacterial cultures accounted for 92 %, 67 %, 57 %, and 52 % under Cd<sup>2+</sup> stress of 0.5, 10, 25, and 50 mg/L, respectively, which were superior to that of individual strains. This study confirmed the potential application and technical reference of the specific mixed bacterial cultures in the enhanced bioremediation of PHE-Cd<sup>2+</sup> co-contamination.</p></div>\",\"PeriodicalId\":8766,\"journal\":{\"name\":\"Biochemical Engineering Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemical Engineering Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1369703X24002201\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1369703X24002201","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Enhanced removal of PHE-Cd2+ co-contamination by the mixed bacterial cultures of Pseudomonas putida and Arthrobacter sp.: Performance and mechanism
The strain species resource is essential for microbial remediation of PHE (phenanthrene)-Cd2+ co-contamination. In this study, the mixed bacterial culture (M) was established to intensively remediate PHE-Cd2+ co-contamination using PAHs-degrading bacteria Pseudomonas putida and Arthrobacter sp. with different Cd2+ tolerance. The PHE degradation efficiency of the mixed bacterial cultures (M) was 65 %-81 % under different Cd2+ concentration of 10–50 mg/L, respectively, which was 1.40–2.98 times that of the individual strains. In addition, strain metabolic enzyme activity, intracellular structure and extracellular polymeric substances (EPS) were carried out under Cd2+ stress. Results showed that the catechol 1,2-dioxygenase enzyme (C120) and the electron transport system activity (ETSA) were less adversely affected by Cd2+ in mixed bacterial cultures. Transmission electron microscopy images showed that the cells surface of the mixed bacterial cultures (M) could be adsorbed more Cd2+ compared to the single strain. The analysis of cell functional groups suggested that C-O-C and C-O groups in EPS mediated the removal of Cd2+ in the mixed bacterial culture. Moreover, the Cd2+ removal proportion by EPS adsorption of the mixed bacterial cultures accounted for 92 %, 67 %, 57 %, and 52 % under Cd2+ stress of 0.5, 10, 25, and 50 mg/L, respectively, which were superior to that of individual strains. This study confirmed the potential application and technical reference of the specific mixed bacterial cultures in the enhanced bioremediation of PHE-Cd2+ co-contamination.
期刊介绍:
The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology.
The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields:
Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics
Biosensors and Biodevices including biofabrication and novel fuel cell development
Bioseparations including scale-up and protein refolding/renaturation
Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells
Bioreactor Systems including characterization, optimization and scale-up
Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization
Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals
Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release
Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites
Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation
Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis
Protein Engineering including enzyme engineering and directed evolution.