Ras 促进果蝇卵巢生殖干细胞分裂

IF 5.9 2区 医学 Q1 CELL & TISSUE ENGINEERING
Stem Cell Reports Pub Date : 2024-08-13 Epub Date: 2024-07-18 DOI:10.1016/j.stemcr.2024.06.005
Qi Zhang, Yanfang Wang, Zhenan Bu, Yang Zhang, Qian Zhang, Le Li, Lizhong Yan, Yuejia Wang, Shaowei Zhao
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引用次数: 0

摘要

Ras 家族基因是原癌基因,从果蝇到人类都高度保守。在果蝇中,RasV12是Ras肿瘤蛋白的组成激活形式,它在细胞周期进展中的功能取决于具体情况。然而,它如何影响雌性生殖干细胞(GSCs)的细胞周期仍是未知数。利用野生型生殖干细胞和bam突变型生殖干细胞样细胞作为模型系统,我们确定RasV12过表达促进生殖干细胞分裂,而不是生长,这与体细胞翼盘细胞的情况相反。Ras通过激活丝裂原活化蛋白激酶(MAPK)信号来实现这一功能。这种信号在有丝分裂生殖细胞的 M 期被特异性激活,包括野生型 GSC 和 bam 突变体 GSC 样细胞。此外,RasV12 的过表达会通过诱导有丝分裂应激反应引发多倍体哺育细胞死亡。鉴于果蝇和哺乳动物GSC之间的相似性,我们认为Ras/MAPK信号也会促进哺乳动物GSC的分裂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ras promotes germline stem cell division in Drosophila ovaries.

The Ras family genes are proto-oncogenes that are highly conserved from Drosophila to humans. In Drosophila, RasV12 is a constitutively activated form of the Ras oncoprotein, and its function in cell-cycle progression is context dependent. However, how it influences the cell cycle of female germline stem cells (GSCs) still remains unknown. Using both wild-type GSCs and bam mutant GSC-like cells as model systems, here we determined that RasV12 overexpression promotes GSC division, not growth, opposite to that in somatic wing disc cells. Ras performs this function through activating the mitogen-activated protein kinase (MAPK) signaling. This signaling is activated specifically in the M phase of mitotic germ cells, including both wild-type GSCs and bam mutant GSC-like cells. Furthermore, RasV12 overexpression triggers polyploid nurse cells to die through inducing mitotic stress. Given the similarities between Drosophila and mammalian GSCs, we propose that the Ras/MAPK signaling also promotes mammalian GSC division.

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来源期刊
Stem Cell Reports
Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY
CiteScore
10.50
自引率
1.70%
发文量
200
审稿时长
28 weeks
期刊介绍: Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.
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