{"title":"腐殖酸对 Achromobacter sp.","authors":"Mengyao Gui","doi":"10.1016/j.jbiosc.2024.06.008","DOIUrl":null,"url":null,"abstract":"<div><p><span>Humic acid<span> (HA), a common natural organic matter, could affect conventional anoxic denitrification. Aim of this study was to investigate effect of HA on the process of aerobic denitrification in </span></span><span><span>Achromobacter</span></span> sp. GAD-3, an aerobic denitrifying strain. The findings demonstrated that an increase in HA concentrations (≥5 mg L<sup>−1</sup>) promoted the aerobic denitrification process (excluding N<sub>2</sub>O reduction), manifesting as higher rates of nitrate removal (6.67–11.1 mg L<sup>−1</sup> h<sup>−1</sup>) and lower levels of nitrite accumulation (30.2–20.7 mg L<sup>−1</sup><span><span>). This was attributed to the increased electron transfer activities and denitrifying </span>reductase activities (including NAR, NIR and NOR) facilitated by HA. Accordingly, the expression of denitrification genes such as </span><span><em>napA</em></span>, <em>cnorB</em>, and <em>nirS</em> was enhanced by HA. Nonetheless, the <em>nosZ</em> gene and N<sub>2</sub>OR activity underwent suppression by HA, which was accountable for N<sub>2</sub><span>O emission. It is crucial to understand the HA mechanism towards aerobic denitrifiers<span> for wastewater treatment plants to enhance nitrogen removal.</span></span></p></div>","PeriodicalId":15199,"journal":{"name":"Journal of bioscience and bioengineering","volume":"138 4","pages":"Pages 338-344"},"PeriodicalIF":2.3000,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of humic acid on aerobic denitrification by Achromobacter sp. strain GAD-3\",\"authors\":\"Mengyao Gui\",\"doi\":\"10.1016/j.jbiosc.2024.06.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Humic acid<span> (HA), a common natural organic matter, could affect conventional anoxic denitrification. Aim of this study was to investigate effect of HA on the process of aerobic denitrification in </span></span><span><span>Achromobacter</span></span> sp. GAD-3, an aerobic denitrifying strain. The findings demonstrated that an increase in HA concentrations (≥5 mg L<sup>−1</sup>) promoted the aerobic denitrification process (excluding N<sub>2</sub>O reduction), manifesting as higher rates of nitrate removal (6.67–11.1 mg L<sup>−1</sup> h<sup>−1</sup>) and lower levels of nitrite accumulation (30.2–20.7 mg L<sup>−1</sup><span><span>). This was attributed to the increased electron transfer activities and denitrifying </span>reductase activities (including NAR, NIR and NOR) facilitated by HA. Accordingly, the expression of denitrification genes such as </span><span><em>napA</em></span>, <em>cnorB</em>, and <em>nirS</em> was enhanced by HA. Nonetheless, the <em>nosZ</em> gene and N<sub>2</sub>OR activity underwent suppression by HA, which was accountable for N<sub>2</sub><span>O emission. It is crucial to understand the HA mechanism towards aerobic denitrifiers<span> for wastewater treatment plants to enhance nitrogen removal.</span></span></p></div>\",\"PeriodicalId\":15199,\"journal\":{\"name\":\"Journal of bioscience and bioengineering\",\"volume\":\"138 4\",\"pages\":\"Pages 338-344\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-07-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of bioscience and bioengineering\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1389172324001877\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of bioscience and bioengineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1389172324001877","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
腐殖酸（HA）是一种常见的天然有机物，可影响常规缺氧反硝化过程。本研究旨在探讨腐殖酸对好氧反硝化菌株 Achromobacter sp.研究结果表明，增加 HA 浓度（≥5 毫克/升-1）可促进需氧反硝化过程（不包括氧化亚氮还原），表现为硝酸盐去除率提高（6.67-11.1 毫克/升-1 小时-1），亚硝酸盐积累水平降低（30.2-20.7 毫克/升-1）。这归因于 HA 促进了电子传递活性和反硝化还原酶活性（包括 NAR、NIR 和 NOR）的提高。因此，HA 提高了 napA、cnorB 和 nirS 等反硝化基因的表达。然而，HA抑制了nosZ基因和N2OR活性，而这正是N2O排放的原因。了解 HA 对污水处理厂好氧脱氮机的作用机制对提高脱氮效果至关重要。
Effect of humic acid on aerobic denitrification by Achromobacter sp. strain GAD-3
Humic acid (HA), a common natural organic matter, could affect conventional anoxic denitrification. Aim of this study was to investigate effect of HA on the process of aerobic denitrification in Achromobacter sp. GAD-3, an aerobic denitrifying strain. The findings demonstrated that an increase in HA concentrations (≥5 mg L−1) promoted the aerobic denitrification process (excluding N2O reduction), manifesting as higher rates of nitrate removal (6.67–11.1 mg L−1 h−1) and lower levels of nitrite accumulation (30.2–20.7 mg L−1). This was attributed to the increased electron transfer activities and denitrifying reductase activities (including NAR, NIR and NOR) facilitated by HA. Accordingly, the expression of denitrification genes such as napA, cnorB, and nirS was enhanced by HA. Nonetheless, the nosZ gene and N2OR activity underwent suppression by HA, which was accountable for N2O emission. It is crucial to understand the HA mechanism towards aerobic denitrifiers for wastewater treatment plants to enhance nitrogen removal.
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The Journal of Bioscience and Bioengineering is a research journal publishing original full-length research papers, reviews, and Letters to the Editor. The Journal is devoted to the advancement and dissemination of knowledge concerning fermentation technology, biochemical engineering, food technology and microbiology.
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