miR-22 在恶性黑色素瘤细胞的增殖、侵袭和迁移过程中负向调节 NOD 样受体蛋白 3 基因。

IF 1.4 4区 医学 Q3 ALLERGY
Postepy Dermatologii I Alergologii Pub Date : 2024-06-01 Epub Date: 2024-04-25 DOI:10.5114/ada.2024.140521
Hongyan Liu, Wenlian Huang, Xiaoshu Pu, Yi Chen, Yinbin Zheng, Ying Lei, Ting Jiang
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引用次数: 0

摘要

简介:恶性黑色素瘤(MM)是一种侵袭性极强的皮肤肿瘤:材料与方法:以人类 MCs(WM239a)和人类表皮黑色素细胞(HEM)为研究材料。通过 qRT-PCR 检测 miR-22 和 NLRP3 的表达水平。NLRP3 蛋白的表达是通过 Western 印迹(WB)分析确定的。通过细胞计数试剂盒-8(CCK-8)、Transwell 细胞侵袭试验和划痕试验评估了 miR-22 和 NLRP3 对 MCs 增殖、侵袭和迁移的影响:结果:miR-22 在 WM239a 中的表达明显低于在 HEM 中的表达。结果:miR-22 在 WM239a 中的表达明显低于 HEM,而 miR-22 在 WM239a 中的表达上调则明显提高了 miR-22、Caspase-1、E-cadherin 的表达和 WM239a 的凋亡率,但白细胞介素-1β(IL-1β)和 NLRP3 的水平、细胞增殖活性、侵袭和迁移能力明显下降。miR-22对NLRP3的负调控可能在MM的活动中发挥了重要作用:进一步的研究将有助于揭示这一调控机制的分子细节,并提供新的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
miR-22 negatively regulating NOD-like receptor protein 3 gene in the proliferation, invasion, and migration of malignant melanoma cells.

Introduction: Malignant melanoma (MM) is a highly aggressive skin tumour.

Aim: To investigate whether miR-22 is involved in the proliferation, invasion, and migration of melanoma cells (MCs) by negatively regulating NOD-like receptor protein 3 (NLRP3) gene.

Material and methods: Human MCs (WM239a) and human epidermal melanocytes (HEM) were used as study material. The expression levels of miR-22 and NLRP3 were detected by qRT-PCR. The expression of NLRP3 protein was determined by Western blot (WB) analysis. The effects of miR-22 and NLRP3 on the proliferation, invasion, and migration of MCs were evaluated by cell counting kit-8 (CCK-8), Transwell cell invasion assay, and scratch assay.

Results: The expression of miR-22 was clearly lower in WM239a than in HEM. Up-regulation of miR-22 expression in WM239a clearly raised the expression of miR-22, Caspase-1, and E-cadherin and the apoptotic rate of WM239a; however, the levels of interleukin-1β (IL-1β) and NLRP3, cell proliferation activity, invasion and migration ability were clearly decreased. The negative regulation of NLRP3 by miR-22 may play a major role in activities of MM.

Conclusions: Further studies will help to reveal the molecular details of this regulatory mechanism and provide new therapeutic strategies.

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来源期刊
CiteScore
2.60
自引率
7.10%
发文量
107
审稿时长
6-12 weeks
期刊介绍: Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii is a bimonthly aimed at allergologists and dermatologists.
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