具有更高的稳定性、水溶性和抗菌性的日硫代硫酸酯/聚氧乙烯共轭 pH 响应胶束的制备和生化评估

IF 2.2 4区 化学 Q3 CHEMISTRY, PHYSICAL
Souptik Bhattacharya, Sayamdipta DasChowdhury
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引用次数: 0

摘要

硫代硫酸二烯丙酯(DT)是一种主要的有机硫化学品,具有多种显著的治疗特性,但它极不稳定且容易降解,这限制了它在生物制药商品中的广泛应用。因此,我们利用聚氧乙烯(Brij S20 和 Brij 58)的自组装特性,设计了适当的 pH 响应胶束载体系统,以在保持 DT 的数量和生物活性的同时,夹带 DT 并提高其在环境温度(25 °C)下的稳定性。与 Brij S20 和 Brij 58 胶束载体系统相比,后者显示出更高的稳定性和对 DT 的吸附能力。此外,研究还发现 DT 在胶束化条件下的稳定性受到 pH 值和温度的显著影响(p < 0.05)。胶束系统能够显著减少降解。此外,pH 值为 1.5 的酸性环境对 DT 从胶束中的释放有很大帮助。大约 77% 的 DT 从 Brij 58 系统中释放出来。进行了各种生化分析。利用较低的 pH 值作为刺激,以可控方式从胶束中释放 DT,这可能会促进 DT 在个人胃肠道腔内或 pH 值较低的癌细胞环境附近的生物作用。此外,还要确保胶束化方法不会损害 DT 的生物活性或降低适当的生物相容性。目前的研究提高了开发出商用 DT 胶束制剂的可能性,该制剂可应用于生物制药和食品相关行业。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Preparation and biochemical evaluation of daily-thiosulfinate/polyoxyethylene conjugated pH-responsive micelle with enhanced stability, hydrosolubility and antibacterial properties

Preparation and biochemical evaluation of daily-thiosulfinate/polyoxyethylene conjugated pH-responsive micelle with enhanced stability, hydrosolubility and antibacterial properties

Preparation and biochemical evaluation of daily-thiosulfinate/polyoxyethylene conjugated pH-responsive micelle with enhanced stability, hydrosolubility and antibacterial properties

Diallyl thiosulfinate (DT), a major organosulfur chemical with several notable therapeutic characteristics, is highly unstable and easily degradable which restricts its extensive use in biopharmaceutical commodities. Therefore, utilizing the self-assembly nature of polyoxyethylene (Brij S20 and Brij 58), appropriate pH-responsive micelle carrier systems have been designed to entrap and improve DT’s stability at an ambient temperature (25 °C) while preserving its quantity and biological activity. Comparing with the Brij S20 with the Brij 58 micelle carrier system, the latter demonstrated superior stability and entrapment of DT. In addition, it was found that DT’s stability in micellized condition is significantly influenced by both pH and temperature (p < 0.05). The micelle system was capable enough to reduce degradation significantly. Additionally, the liberation of DT from micelle is greatly aided by acidic pH 1.5. Around 77% DT was released from Brij 58 system. Various biochemical analyses were done. The liberation of DT from the micelle in a controlled manner using lower pH as stimuli may facilitate its biological action at an individual’s gastrointestinal lumen or near cancer cell environment having lower pH. Additionally, it was made sure that the micellization method did not impair DT’s bioactivity or reduce appropriate biocompatibility. The current study increases the likelihood of creating a commercially available DT-loaded, micelle-based formulation for application in biopharma and food-related industries.

Graphical Abstract

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来源期刊
Colloid and Polymer Science
Colloid and Polymer Science 化学-高分子科学
CiteScore
4.60
自引率
4.20%
发文量
111
审稿时长
2.2 months
期刊介绍: Colloid and Polymer Science - a leading international journal of longstanding tradition - is devoted to colloid and polymer science and its interdisciplinary interactions. As such, it responds to a demand which has lost none of its actuality as revealed in the trends of contemporary materials science.
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