{"title":"西波姬鱼甲壳素及其抑制血液凝固的能力和对人类病原体的抗菌作用","authors":"","doi":"10.1016/j.procbio.2024.07.016","DOIUrl":null,"url":null,"abstract":"<div><p>Chitosan, a derivative of chitin derived from the gladius of the marine cephalopod <em>Doryteuthis sibogae</em>, has drawn interest in biomedical research because of its possible antibacterial effects on human infections and inhibition of blood clotting. Chitosan was extracted from chitin isolated from the gladius of <em>D. sibogae</em> via demineralization and deproteination. Chitosan was structurally characterized using FT-IR, XRD, and FESEM. Additionally, the disc diffusion method was used to investigate the antibacterial efficacy of chitosan against three human pathogens. Effective antibacterial activity was demonstrated against both Gram-positive and Gram-negative microorganisms. Chitosan is a potent anticoagulant in anticoagulant assays employing activated partial thromboplastin time (APTT) and prothrombin time (PT).</p></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chitosan from gladius of Doryteuthis sibogae and their capability to inhibit the blood clotting and its antibacterial effect against human pathogens\",\"authors\":\"\",\"doi\":\"10.1016/j.procbio.2024.07.016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Chitosan, a derivative of chitin derived from the gladius of the marine cephalopod <em>Doryteuthis sibogae</em>, has drawn interest in biomedical research because of its possible antibacterial effects on human infections and inhibition of blood clotting. Chitosan was extracted from chitin isolated from the gladius of <em>D. sibogae</em> via demineralization and deproteination. Chitosan was structurally characterized using FT-IR, XRD, and FESEM. Additionally, the disc diffusion method was used to investigate the antibacterial efficacy of chitosan against three human pathogens. Effective antibacterial activity was demonstrated against both Gram-positive and Gram-negative microorganisms. Chitosan is a potent anticoagulant in anticoagulant assays employing activated partial thromboplastin time (APTT) and prothrombin time (PT).</p></div>\",\"PeriodicalId\":20811,\"journal\":{\"name\":\"Process Biochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-07-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Process Biochemistry\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1359511324002381\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Process Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1359511324002381","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
壳聚糖是从海洋头足类动物 Doryteuthis sibogae 的甲壳质中提取的一种衍生物,由于其可能对人类感染具有抗菌作用并能抑制血液凝结,因此引起了生物医学研究的兴趣。壳聚糖是通过脱矿物质和脱蛋白的方法从 D. sibogae 角叉鱼的甲壳素中提取出来的。利用傅立叶变换红外光谱(FT-IR)、X射线衍射(XRD)和FESEM对壳聚糖进行了结构表征。此外,还使用圆盘扩散法研究了壳聚糖对三种人类病原体的抗菌效果。结果表明,壳聚糖对革兰氏阳性和革兰氏阴性微生物都具有有效的抗菌活性。在采用活化部分凝血活酶时间(APTT)和凝血酶原时间(PT)的抗凝试验中,壳聚糖是一种强效抗凝剂。
Chitosan from gladius of Doryteuthis sibogae and their capability to inhibit the blood clotting and its antibacterial effect against human pathogens
Chitosan, a derivative of chitin derived from the gladius of the marine cephalopod Doryteuthis sibogae, has drawn interest in biomedical research because of its possible antibacterial effects on human infections and inhibition of blood clotting. Chitosan was extracted from chitin isolated from the gladius of D. sibogae via demineralization and deproteination. Chitosan was structurally characterized using FT-IR, XRD, and FESEM. Additionally, the disc diffusion method was used to investigate the antibacterial efficacy of chitosan against three human pathogens. Effective antibacterial activity was demonstrated against both Gram-positive and Gram-negative microorganisms. Chitosan is a potent anticoagulant in anticoagulant assays employing activated partial thromboplastin time (APTT) and prothrombin time (PT).
期刊介绍:
Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.