各种抗氧化剂对绵羊卵巢组织玻璃化成功率的影响

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2024-07-01 DOI:10.54680/fr24410110212

Masrat un Nisa, A. Malik, Khursheed Ahmad Sofi, Beenish Qureshi, A. Akand, Showkat Ahmad Shah, Tanveer Mushtaq, Nahida Yousuf, Pawanpreet Singh

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引用次数: 0

摘要

背景玻璃化是一种冷冻保存技术，被认为是保存卵母细胞、胚胎和性腺组织的一种有前途的替代方法。目的确定不同抗氧化剂对卵巢组织解冻后存活率、取回卵母细胞的形态学和玻璃化卵巢组织的组织学的影响、白藜芦醇（20 uM）、ZnSO4（500 uM）、姜黄素（25 uM）和槲皮素（1 uM）]加入玻璃化和升温培养基后，评估了它们对取回卵母细胞的存活率和形态以及玻璃化卵巢组织的组织学的影响。其中，上述不同抗氧化剂和玻璃化对照组的存活卵母细胞数分别为 24 个（70.6%）、30 个（73.1%）、20 个（76.9%）、26 个（83.9%）和 33 个（71.7%），形态正常的卵母细胞数分别为 24 个（70.6%）、26 个（63.4%）、18 个（69.2%）、21 个（67.7%）和 34 个（73.9%）。不同处理组之间的差异不显著（P. > 0.05）。卵巢皮质组织的组织形态学评估显示，新鲜对照组完整卵泡的百分比（84.19±3.9）明显高于其他组（P<0.05）。白藜芦醇组（50.2±5.5）、姜黄素组（48.7±5.7）、槲皮素组（51.6±4.8）和玻璃化对照组（42.7±6.1）之间差异不显著；然而，补充 ZnSO4 组（23.1±8.54）与其他抗氧化剂组差异显著（P < 0.结论添加这些浓度的抗氧化剂白藜芦醇、姜黄素和槲皮素往往能非显著性地改善玻璃化后卵泡的完整性。Doi.org/10.54680/fr24410110212.

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Effect of various antioxidants on ovarian tissue vitrification success in sheep.

BACKGROUND Vitrification is a technique of cryopreservation that has been proposed as a promising alternative method for the preservation of oocytes, embryos and gonadal tissue. OBJECTIVE To determine the effect of different antioxidants on post-thaw viability, morphology of retrieved oocytes and histology of vitrified ovarian tissue. MATERIALS AND METHODS Four different antioxidants [i.e., resveratrol (20 uM), ZnSO4 (500 uM), curcumin (25 uM) and quercetin (1 uM)] were evaluated after their addition to the vitrification and warming media for their effects on the viability and morphology of retrieved oocytes and the histology of vitrified ovarian tissue. RESULTS The number of oocytes retrieved from ovarian tissue from the above mentioned antioxidants and vitrified control were 34, 41, 26, 31 and 46 respectively. Among these the number of viable oocytes were found to be 24 (70.6%), 30 (73.1 %), 20 (76.9%), 26 (83.9%) and 33 (71.7%) and the number of oocytes found morphologically normal were 24 (70.6%), 26 (63.4%), 18 (69.2%), 21 (67.7%) and 34 (73.9%) for the above mentioned different antioxidants and vitrified control, respectively. Non-significant (P. > 0.05) differences were found between different treatment groups. Histomorphological evaluation of the ovarian cortical tissue showed that the percentage of intact follicles was significantly (P < 0.05) higher in the fresh control (84.19±3.9) than in other groups. Non-significant differences were found between resveratrol (50.2±5.5), curcumin (48.7±5.7), quercetin (51.6±4.8) and the vitrified control (42.7±6.1) groups; however, the ZnSO4 supplemented group (23.1±8.54) differed significantly (P < 0.05) from other antioxidant groups but was non-significant (P > 0.05) with the vitrified control group (42.7±6.1). CONCLUSION The addition of antioxidants resveratrol, curcumin and quercetin at these concentrations tended to non-significantly improve the follicular integrity after vitrification. Doi.org/10.54680/fr24410110212.

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.